The Saccharomyces cerevisiae TGL2 gene encodes a protein with lipolytic activity and can complement an Escherichia coli diacylglycerol kinase disruptant

Yeast
G P van HeusdenH Y Steensma

Abstract

Escherichia coli cells with a disrupted diacylglycerol kinase gene are unable to grow on media containing arbutin due to a lethal accumulation of diacylglycerol. In order to isolate genes from the yeast Saccharomyces cerevisiae involved in diacylglycerol metabolism we complemented an E. coli diacylglycerol kinase disruptant with a yeast genomic library and transformants were selected capable of growing in the presence of arbutin. Using this method, a gene (TGL2) was isolated coding for a protein resembling lipases from Pseudomonas. After expression of the TGL2 gene in E. coli, lipolytic activity towards triacylglycerols and diacylglycerols with short-chain fatty acids could be measured. Therefore, it is very likely that the TGL2 gene can complement the E. coli diacylglycerol kinase disruptant, because it encodes a protein that degrades the diacylglycerol accumulated after growth in the presence of arbutin. Disruption of the TGL2 gene in S. cerevisiae did not result in a detectable phenotype. The role of the Tgl2 protein in lipid degradation in yeast is still unclear.

References

Sep 1, 1992·Molecular Plant-microbe Interactions : MPMI·K J MillerB T Nixon
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S W RamerR W Davis
Dec 1, 1992·Applied and Environmental Microbiology·L G FrenkenC T Verrips
Jan 1, 1991·Annual Review of Genetics·D M Nikoloff, S A Henry
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J ElledgeR W Davis
Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H S SeifertF Heffron
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Aug 1, 1970·The Biochemical Journal·T Nurminen, H Suomalainen
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jan 1, 1993·Trends in Biochemical Sciences·Z S Derewenda, A M Sharp
Feb 1, 1996·Plant Molecular Biology·T KatagiriK Shinozaki
Aug 16, 1996·The Journal of Biological Chemistry·T M KlauckS Jaken

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Citations

Dec 5, 2009·The Journal of Biological Chemistry·Hye Jin HamHye-Joo Yoon
Feb 9, 2008·Progress in Lipid Research·Sona RajakumariGünther Daum
Sep 28, 2012·The FEBS Journal·Minh T TaHongyuan Yang
Aug 19, 2006·Biochimica Et Biophysica Acta·Tibor CzabanyGünther Daum
May 14, 2003·Applied Microbiology and Biotechnology·D Sorger, G Daum
Apr 19, 2003·The Journal of Biological Chemistry·Karin Athenstaedt, Gunther Daum
Sep 5, 2019·Molecular Biology of the Cell·Fenja OdendallKai Stefan Dimmer
Jun 7, 2019·Biotechnology for Biofuels·Ievgeniia A TiukovaEduard J Kerkhoven

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