The saposin-like proteins 1, 2, and 3 of Fasciola gigantica

Molecular and Biochemical Parasitology
Rudi GramsVithoon Viyanant

Abstract

The SAP genes of Fasciola encode proteins belonging to the saposin-like protein family. The saposin signature, a compact domain of mainly alpha-helical character, contains six conserved cysteine residues and has been implicated in membrane-binding, pore formation, and subsequent cell lysis in several family members. Recombinant SAP-2 of F. hepatica has been shown to induce lysis of human erythrocytes and peripheral blood mononuclear cells. This suggests that the SAPs are involved in the nutrition of Fasciola as the released content of lysed host cells is available for further enzymatic processing and uptake by the parasite. In the present study a new SAP-3 cDNA was obtained in an immunoscreen of an adult stage F. gigantica cDNA library with an antiserum against the parasite's excretion/secretion antigens. SAP-1 and SAP-2 cDNAs were isolated from F. gigantica cDNA libraries using oligonucleotide primers specific to the SAP-1 and SAP-2 DNA sequences from F. hepatica. Transcripts of the three SAPs are present from the metacercarial to the adult stage and are located to the gut epithelium. In immatures SAP-1 RNA is the predominant product whereas in adults SAP-2 and -3 are the more abundant products. Polyclonal anti-SAP-1 and SAP-2...Continue Reading

Citations

Dec 29, 2010·Parasitology Research·Pornanan KueakhaiPrasert Sobhon
Jun 17, 2010·Parasitology·Supatra ChunchobSuksiri Vichasri-Grams
Aug 31, 2013·Acta Tropica·Kimberly Cabán-Hernández, Ana M Espino
May 7, 2009·Molecular and Biochemical Parasitology·Mayuri TarasukRudi Grams
Jun 24, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Tegan A DonAlex Loukas
Nov 26, 2008·Fish & Shellfish Immunology·M Prado-AlvarezA Figueras
Sep 8, 2016·Scientific Reports·Lucía Sánchez Di MaggioPatricia Berasain

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