PMID: 9444965Jan 28, 1998Paper

The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity

Life Sciences
E C SamouilidouJ T Darsinos

Abstract

This study was designed to examine the role of calcium in the ischemia-induced changes of calmodulin-stimulated Ca2+-ATPase activity of heart sarcolemma of dogs subjected to coronary artery ligation (90 min) and reperfusion (30 min). This was attained by the application of systemic hemodialysis with low Ca2+ dialysate in six dogs (group A) and the comparison of the results with those obtained from animals subjected to normal Ca2+ hemodialysis (control group B, n=7). A very significant (p<0.001) decrease was found in the calmodulin-stimulated Ca2+-ATPase activity measured in the ischemic and non-ischemic parts of group B. This was associated with a decrease in the maximal velocity (v(max)) of the reaction of stimulation and an increase in the apparent Km for calmodulin. The kinetics of the calmodulin-stimulated Ca2+-ATPase also assessed in the presence of trifluoroperazine, a specific inhibitor for calmodulin binding, showed that the affinity for calmodulin was higher in the ischemic part of group A than of B, while v(max) was not substantially different. The above data may suggest that the inhibition of the calmodulin-stimulated Ca2+-ATPase produced by ischemia-reperfusion and its preservation under low Ca2+, are exerted at the...Continue Reading

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Citations

Mar 29, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li ZhangHuajin Liu
Nov 23, 2006·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti

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