The SARS coronavirus nucleocapsid protein--forms and functions

Antiviral Research
Chung-ke ChangTai-huang Huang

Abstract

The nucleocapsid phosphoprotein of the severe acute respiratory syndrome coronavirus (SARS-CoV N protein) packages the viral genome into a helical ribonucleocapsid (RNP) and plays a fundamental role during viral self-assembly. It is a protein with multifarious activities. In this article we will review our current understanding of the N protein structure and its interaction with nucleic acid. Highlights of the progresses include uncovering the modular organization, determining the structures of the structural domains, realizing the roles of protein disorder in protein-protein and protein-nucleic acid interactions, and visualizing the ribonucleoprotein (RNP) structure inside the virions. It was also demonstrated that N-protein binds to nucleic acid at multiple sites with a coupled-allostery manner. We propose a SARS-CoV RNP model that conforms to existing data and bears resemblance to the existing RNP structures of RNA viruses. The model highlights the critical role of modular organization and intrinsic disorder of the N protein in the formation and functions of the dynamic RNP capsid in RNA viruses. This paper forms part of a symposium in Antiviral Research on "From SARS to MERS: 10 years of research on highly pathogenic human ...Continue Reading

References

Feb 24, 1979·The Veterinary Record·E O Caul, S I Egglestone
Jun 1, 1978·The Journal of General Virology·M R Macneughton, H A Davies
Mar 1, 1981·The Journal of General Virology·H A DaviesM R Macnaughton
May 9, 2000·Journal of Virology·C A de HaanP J Rottier
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A ShoemakerP G Wolynes
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
May 23, 2002·Biochemistry·A Keith DunkerZoran Obradović
May 6, 2003·Science·Marco A MarraRachel L Roper
Mar 17, 2004·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
Apr 20, 2004·Biochemical and Biophysical Research Communications·Milan SurjitSunil K Lal
May 19, 2004·Biochemistry·Qiulong HuangEdward T Olejniczak
Dec 8, 2004·Current Topics in Medicinal Chemistry·Rabi Ann Musah
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Oct 18, 2005·Journal of Biomedical Science·Chung-ke ChangTai-huang Huang
Jun 17, 2006·Science·Aurélie A V AlbertiniRob W H Ruigrok
Sep 29, 2006·Journal of Molecular Recognition : JMR·Adessamad Ababou, John E Ladbury
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vincent J Hilser, E Brad Thompson
Sep 21, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Milan Surjit, Sunil K Lal
Feb 5, 2008·Biochemical and Biophysical Research Communications·Yingchun ZengZhenghui Wu

❮ Previous
Next ❯

Citations

Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Mar 24, 2017·Journal of Virology·Bozena SzelazekGrzegorz Dubin
Sep 9, 2017·Oncotarget·Jidang Chen, Hinh Ly
Apr 30, 2020·Journal of the Chinese Medical Association : JCMA·Tai-Jay ChangChin-Tien Wang
Jun 4, 2020·Journal of Medical Virology·Domenico BenvenutoAntonio Cassone
Jun 13, 2020·Expert Review of Respiratory Medicine·Arafat HussainAnant Mohan
Aug 5, 2020·Archives of Microbiology·Muhammad Tahir KhanMuhammad Irfan
Apr 30, 2020·Journal of the Chinese Medical Association : JCMA·Kung-Hao LiangDe-Ming Yang
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Yong Sheng WangMing Hon Hou
Feb 20, 2016·Acta Crystallographica. Section D, Structural Biology·Nicolas PapageorgiouBruno Coutard
Feb 25, 2017·Intrinsically Disordered Proteins·Shelly DeForteVladimir N Uversky
Jul 20, 2017·Scientific Reports·Yingying CongFulvio Reggiori
Jun 18, 2020·Journal of Virology·Noton K DuttaJames T Gordy
Jan 16, 2019·Acta Crystallographica. Section D, Structural Biology·Thi Hong Van NguyenBruno Coutard
Jun 14, 2020·Signal Transduction and Targeted Therapy·Bin ChenWei Cheng
May 27, 2020·Virologica Sinica·Zhiqiang ChenJianfeng Dai
Mar 24, 2020·Indian Journal of Pharmacology·Manisha PrajapatBikash Medhi
Aug 11, 2020·Journal of the Chinese Medical Association : JCMA·Ping-Hsing TsaiTai-Jay Chang
Sep 26, 2020·Frontiers in Molecular Biosciences·Eleni Nikolakaki, Thomas Giannakouros
May 3, 2020·Emerging Microbes & Infections·Baoqing SunLing Chen
Aug 6, 2020·Frontiers in Medicine·Sama RezasoltaniMohammad Reza Zali
Aug 7, 2020·Chemical Communications : Chem Comm·Liyun ZhangZhaofeng Luo
Jul 29, 2020·International Journal of Environmental Research and Public Health·Lisa CarianiGiovanna Lunghi
Aug 4, 2020·Infectious Diseases of Poverty·Hong-Zhi ChenXiang Wu

❮ Previous
Next ❯

Methods Mentioned

BETA
electron cryo-microscopy
X-ray
NMR
size-exclusion chromatography

Software Mentioned

ClustalOmega
TGEV
PyMOL Molecular Graphics System
PyMOL

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.