The SARS coronavirus S glycoprotein receptor binding domain: fine mapping and functional characterization

Virology Journal
Samitabh ChakrabortiDimiter S Dimitrov

Abstract

The entry of the SARS coronavirus (SCV) into cells is initiated by binding of its spike envelope glycoprotein (S) to a receptor, ACE2. We and others identified the receptor-binding domain (RBD) by using S fragments of various lengths but all including the amino acid residue 318 and two other potential glycosylation sites. To further characterize the role of glycosylation and identify residues important for its function as an interacting partner of ACE2, we have cloned, expressed and characterized various soluble fragments of S containing RBD, and mutated all potential glycosylation sites and 32 other residues. The shortest of these fragments still able to bind the receptor ACE2 did not include residue 318 (which is a potential glycosylation site), but started at residue 319, and has only two potential glycosylation sites (residues 330 and 357). Mutation of each of these sites to either alanine or glutamine, as well as mutation of residue 318 to alanine in longer fragments resulted in the same decrease of molecular weight (by approximately 3 kDa) suggesting that all glycosylation sites are functional. Simultaneous mutation of all glycosylation sites resulted in lack of expression suggesting that at least one glycosylation site (...Continue Reading

References

Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
May 16, 2003·The New England Journal of Medicine·Kathryn V Holmes
Jun 26, 2003·Nucleic Acids Research·Ramu ChennaJulie D Thompson
Dec 4, 2003·Biochemical and Biophysical Research Communications·Xiaodong XiaoDimiter S Dimitrov
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Dec 17, 2003·Cell·Dimiter S Dimitrov
Jan 13, 2004·Biochemical and Biophysical Research Communications·Ponraj PrabakaranDimiter S Dimitrov
Feb 3, 2004·The Journal of Biological Chemistry·Paul TowlerMichael W Pantoliano
Feb 10, 2004·Biochemical and Biophysical Research Communications·Peigang WangHongkui Deng
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianhua SuiWayne A Marasco
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates
Mar 27, 2004·Nature Reviews. Microbiology·Dimiter S Dimitrov
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himani BishtBernard Moss
Jun 25, 2004·Nucleic Acids Research·Scott McGinnis, Thomas L Madden
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan

❮ Previous
Next ❯

Citations

Nov 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michelle M BeckerMark R Denison
Jun 15, 2007·Antioxidants & Redox Signaling·Emmanuel FenouilletIan M Jones
May 23, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jonathan M GershoniYael Weiss
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhongyu ZhuDimiter S Dimitrov
Feb 17, 2009·Expert Opinion on Biological Therapy·Ponraj PrabakaranDimiter S Dimitrov
Jun 15, 2007·Antiviral Research·F J U M van der MeerH F Egberink
Jan 31, 2006·Expert Review of Anti-infective Therapy·Zhongyu ZhuDimiter S Dimitrov
Apr 7, 2006·The Journal of Biological Chemistry·Ponraj PrabakaranDimiter S Dimitrov
Dec 27, 2019·Mass Spectrometry Reviews·Aleksandra MilewskaPiotr Suder
Jan 19, 2006·The Journal of Biological Chemistry·Dimitri LavilletteEmmanuel Fenouillet
May 3, 2020·British Journal of Pharmacology·Steve P H AlexanderMichael Spedding
Sep 7, 2006·The Journal of Biological Chemistry·William C HwangRobert C Liddington
Jun 1, 2018·Future Virology·To Sing Fung, Ding Xiang Liu
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeShibo Jiang
Dec 5, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Samuel K LaiRaymond J Pickles
Jan 19, 2021·Computational and Structural Biotechnology Journal·Camila PontesAlfonso Valencia
Sep 29, 2020·Heliyon·Alessandra Lo PrestiPaola Stefanelli

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
glycosylations
PCR
transfection
ELISA

Software Mentioned

ClustalW
InsightII
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.