The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA.

Nature Communications
J. CubukAlex S Holehouse


The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein critical for viral genome packaging, yet the molecular details that underlie this process are poorly understood. Here we combine single-molecule spectroscopy with all-atom simulations to uncover the molecular details that contribute to N protein function. N protein contains three dynamic disordered regions that house putative transiently-helical binding motifs. The two folded domains interact minimally such that full-length N protein is a flexible and multivalent RNA-binding protein. N protein also undergoes liquid-liquid phase separation when mixed with RNA, and polymer theory predicts that the same multivalent interactions that drive phase separation also engender RNA compaction. We offer a simple symmetry-breaking model that provides a plausible route through which single-genome condensation preferentially occurs over phase separation, suggesting that phase separation offers a convenient macroscopic readout of a key nanoscopic interaction.


Apr 1, 1987·Biopolymers·J A Schellman
Jan 1, 1997·Journal of Virology·K WooS Makino
Dec 10, 1999·Journal of Virology·R Cologna, B G Hogue
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Mar 17, 2004·Biochemical and Biophysical Research Communications·Runtao HeXuguang Li
Nov 30, 2005·RNA·Travis S BayerAndrew D Ellington
Sep 19, 2006·Virology·Sonia ZúñigaLuis Enjuanes
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel NettelsBenjamin Schuler
Jul 7, 2007·The Journal of Chemical Physics·René Pool, Peter G Bolhuis
Aug 31, 2007·Science·M Shirts, V S Pande
Dec 7, 2007·Trends in Biochemical Sciences·Peter Tompa, Monika Fuxreiter
May 29, 2008·Journal of Computational Chemistry·Andreas Vitalis, Rohit V Pappu
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daniel NettelsBenjamin Schuler
Aug 27, 2010·Journal of Virology·Monique H VerheijeCornelis A M de Haan
Jan 6, 2011·Protein Science : a Publication of the Protein Society·Cédric LeyratMarc Jamin
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrea SorannoBenjamin Schuler
Jun 12, 2012·Cell·Stephanie C Weber, Clifford P Brangwynne
Aug 18, 2012·The Journal of Chemical Physics·Albert H Mao, Rohit V Pappu
Sep 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hagen HofmannBenjamin Schuler
Feb 21, 2013·RNA Biology·Alexander BorodavkaPeter G Stockley

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Jun 10, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Yamilka DíazUNKNOWN Gorgas COVID19 Team

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