The SARS-CoV S glycoprotein: expression and functional characterization

Biochemical and Biophysical Research Communications
Xiaodong XiaoD S Dimitrov

Abstract

We have cloned, expressed, and characterized the full-length and various soluble fragments of the SARS-CoV (Tor2 isolate) S glycoprotein. Cells expressing S fused with receptor-expressing cells at neutral pH suggesting that the recombinant glycoprotein is functional, its membrane fusogenic activity does not require other viral proteins, and that low pH is not required for triggering membrane fusion; fusion was not observed at low receptor concentrations. S and its soluble ectodomain, S(e), were not cleaved to any significant degree. They ran at about 180-200kDa in SDS gels suggesting post-translational modifications as predicted by previous computer analysis and observed for other coronaviruses. Fragments containing the N-terminal amino acid residues 17-537 and 272-537 but not 17-276 bound specifically to Vero E6 cells and purified soluble receptor, ACE2, recently identified by M. Farzan and co-workers [Nature 426 (2003) 450-454]. Together with data for inhibition of binding by antibodies developed against peptides from S, these findings suggest that the receptor-binding domain is located between amino acid residues 303 and 537. These results also confirm that ACE2 is a functional receptor for the SARS virus and may help in the...Continue Reading

References

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Citations

Jan 13, 2004·Biochemical and Biophysical Research Communications·Ponraj PrabakaranDimiter S Dimitrov
Jun 9, 2005·Protein Expression and Purification·Xun ZuoTauseef R Butt
Aug 5, 2005·Nature Medicine·John Nicholls, Malik Peiris
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Mar 27, 2004·Nature Reviews. Microbiology·Dimiter S Dimitrov
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
Feb 1, 2012·The Journal of Biological Chemistry·Kailang WuFang Li
Jun 15, 2007·Antioxidants & Redox Signaling·Emmanuel FenouilletIan M Jones
Jul 23, 2005·Viral Immunology·Yuxian He, Shibo Jiang
Feb 26, 2005·Bioinformatics·Daniel Shegogue, W Jim Zheng
Jan 25, 2007·Annual Review of Immunology·Jun Chen, Kanta Subbarao
Aug 24, 2005·Genetic Vaccines and Therapy·Ali AziziFrancisco Diaz-Mitoma
Aug 27, 2005·Virology Journal·Samitabh ChakrabortiDimiter S Dimitrov
Mar 25, 2006·Uirusu·Masato Tsurudome
Nov 20, 2004·Emerging Infectious Diseases·M L NgE E Ooi

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Methods Mentioned

BETA
ELISA
PCR
transfection
flow cytometry
transfect
glycosylation

Software Mentioned

MethaMorph

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