The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield

Applied Microbiology and Biotechnology
Anna Bzducha-WróbelMarek Kieliszek

Abstract

New ideas on production of yeast origin β-glucan preparations for industrial application are attracting interest considering market development of that high-value functional polysaccharide. Sellecting an efficient yeast producer and designing culture conditions are a prerequisite for obtaining high yield of β-glucan. The aim of this study was to describe at the first time the influence of the mode of cultivation (shake-flasks and batch fermentation) and time of culture on characteristic and yield of biomass and β(1,3)/(1,6)-glucan preparations of Candida utilis ATCC 9950 after cultivation in medium based on waste potato juice water supplemented with 10% of glycerol. After shake-flask culture, the biomass was characterized by higher protein content (app. 26.5%) compared to 19% after batch fermentation while the cultivation on a biofermentor scale promoted polysaccharides biosynthesis. The highest output of purified β(1,3)/(1,6)-glucan preparation (5.3 gd.w./L), containing app. 85% of that polysaccharide, was found after 48 h cultivation in biofermentor. Batch fermentation promoted biosynthesis of alkali-insoluble β(1,3)/(1,6)-glucan fraction, decreasing the content of β(1,6)-glucan. The yield of β(1,3)/(1,6)-glucan synthesis was...Continue Reading

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Citations

Jul 8, 2020·Biological Trace Element Research·Marek Kieliszek, Marianna Dourou
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Przemysław Łukasz KowalczewskiGrażyna Lewandowicz
May 21, 2019·Open Life Sciences·Przemysław Łukasz KowalczewskiGrażyna Lewandowicz
Apr 6, 2021·Open Life Sciences·Paweł JeżowskiDominika Radzikowska

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Methods Mentioned

BETA
transmission electron microscopy
enzymatic assay
electron microscopy

Software Mentioned

STATISTICA

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