The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast

Nucleic Acids Research
Naglis MalysJohn E G McCarthy

Abstract

Dcs1, the m7GpppX pyrophosphatase of Saccharomyces cerevisiae, has been reported to 'scavenge' capped 5' end fragments generated by 3'-->5' mRNA degradation. We now show that the absence of Dcs1, and the closely related Dcs2 protein, compromises cellular responses to glucose-deprivation stress as well as to step changes in glucose availability. Dcs1 and Dcs2 form homo- and heterodimers, with the heterodimer appearing as cells enter diauxie. Despite the previously observed increase in abundance of the mRNA encoding the neutral trehalase (Nth1) in the stationary phase, the total enzyme activity of Nth1 decreases in this phase of growth. Changes in trehalase activity are significant because the non-reducing disaccharide trehalose is thought to stabilize cellular components under stress conditions. In the dcs1Delta and dcs1Deltadcs2Delta mutants, normal regulation of trehalase activity is lost. Nutrient stress induces DCS1 and DCS2 transcription via the cAMP-PKA signalling pathway. Dcs1 also becomes phosphorylated as the availability of glucose diminishes, and we test the role of this phosphorylation in the stress response. Further evidence indicates that Dcs1 plays a complementary role to the translation factor eIF4E in preventing...Continue Reading

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Citations

Aug 14, 2013·Journal of Biosciences·Satarupa Das, Biswadip Das
May 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Flore SinturelLionel Bénard
Apr 29, 2008·The Journal of Biological Chemistry·Shin-Wu LiuMegerditch Kiledjian
May 8, 2007·Nucleic Acids Research·Padchanee SangthongJohn E G McCarthy
Nov 2, 2005·Molecular and Cellular Biology·Hudan Liu, Megerditch Kiledjian
Aug 10, 2012·BMC Research Notes·Nuno F AzevedoMaria J Vieira
Apr 23, 2008·RNA·Vincent ShenMegerditch Kiledjian
Jul 13, 2012·Genetics·Roy Parker
Oct 4, 2015·Mechanisms of Ageing and Development·Fivos Borbolis, Popi Syntichaki
May 27, 2006·EMBO Reports·Helena HernándezCarol V Robinson
Jan 25, 2011·Molecular Microbiology·Peter LeeJi-Sook Hahn
Sep 12, 2006·Journal of Molecular Biology·Naglis Malys, John E G McCarthy
Apr 20, 2014·Biochimica Et Biophysica Acta·Adina L MilacAnna Wypijewska del Nogal
Jun 11, 2011·FEMS Microbiology Reviews·Claudio De Virgilio
Jan 4, 2008·Physiological Genomics·Anja ByeUlrik Wisløff
Jul 10, 2018·FEMS Yeast Research·Claudio Falcone, Cristina Mazzoni
Oct 23, 2018·Wiley Interdisciplinary Reviews. RNA·Susanne Kramer, Alexander G McLennan
Mar 14, 2018·Cancer Cell·Akihide Yoshimi, Omar Abdel-Wahab

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