The Schizosaccharomyces pombe mei4+ gene encodes a meiosis-specific transcription factor containing a forkhead DNA-binding domain.

Molecular and Cellular Biology
S HorieC Shimoda

Abstract

The mei4+ gene of the fission yeast Schizosaccharomyces pombe was cloned by functional complementation. The mei4 disruptant failed to complete meiosis-I but could proliferate normally. mei4+ was transcribed only in meiosis-proficient diploid cells after premeiotic DNA replication. The mei4+ open reading frame encodes a 57-kDa serine-rich protein comprised of 517 amino acids with a forkhead/HNF3 DNA-binding domain in the amino-terminal region. Transcription of spo6+, a gene required for sporulation, was dependent on the mei4+ function. Two copies of the GTAAAYA consensus sequence, proposed as the binding site for human forkhead proteins, were found in the promoter region of spo6+. A gel mobility shift assay demonstrated the sequence-dependent binding of the GST-Mei4 forkhead domain fusion protein to DNA fragments with one of the consensus elements. Deletion of this consensus element from the spo6 promoter abolished the transcription of spo6+ and resulted in a sporulation deficiency. One-hybrid assay of Mei4 which was fused to the Gal4 DNA-binding domain localized the transcriptional activation domain in the C-terminal 140 amino acids of Mei4. These results indicate that Mei4 functions as a meiosis-specific transcription factor o...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1979·Cell·L HerefordD B Kaback
May 1, 1992·Molecular & General Genetics : MGG·N Mochizuki, M Yamamoto
Apr 1, 1992·Molecular & General Genetics : MGG·E Warbrick, P A Fantes
Mar 22, 1990·Nature·D A HughesM Yamamoto
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T MaedaM Yamamoto
Jun 1, 1990·Canadian Journal of Microbiology·H MiyataB F Johnson
Aug 1, 1974·Journal of General Microbiology·D G Smith
Jan 1, 1968·Molecular & General Genetics : MGG·C BreschR Egel
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Jan 1, 1983·Methods in Enzymology·R J Rothstein
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R JensenI Herskowitz
Sep 1, 1995·Molecular and Cellular Biology·M WillerO Nielsen
Mar 1, 1994·Microbiological Reviews·A P Mitchell
Jan 1, 1996·Trends in Biochemical Sciences·M Yamamoto

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Citations

Feb 11, 2011·Molecular Microbiology·Stefan Irniger
Jan 5, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomohiko MoritaKunihiro Ohta
Feb 11, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mayumi ArataMasayuki Yamamoto
Jun 3, 2008·Current Biology : CB·Luther DavisCristina Martín-Castellanos
Nov 18, 2004·Molecular Cell·Mark R FloryRuedi Aebersold
May 5, 2012·PloS One·Raphael IoannoniSimon Labbe
Nov 26, 2014·Annual Review of Genetics·Scott KeeneyNeeman Mohibullah
Nov 16, 2005·Annual Review of Genetics·Jürg Bähler
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Egel
Aug 28, 2013·Nucleic Acids Research·Akira YamashitaMasayuki Yamamoto
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yuko Murakami-TonamiHiroshi Murakami
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Beate SchwerStewart Shuman
Nov 18, 2011·Biology of Reproduction·Gregory M Buchold
Jun 15, 2004·Nature Genetics·Gabriella RusticiJürg Bähler
Apr 17, 2009·Nature·Takeshi SakunoYoshinori Watanabe
Jul 11, 2000·Nature Genetics·S L Forsburg, J A Hodson
Aug 13, 2011·Molecular Biology of the Cell·Jun KashiwazakiTaro Nakamura
Dec 15, 2010·Molecular Biology of the Cell·Nicole CremonaJo Ann Wise
Mar 14, 2014·Molecular Biology of the Cell·Kana FukunishiTaro Nakamura
Oct 11, 2003·Molecular Biology of the Cell·Michael G Catlett, Susan L Forsburg
Nov 11, 2017·Cell Discovery·Vera MoiseevaKazunori Tomita
Jul 11, 2006·Nature·Yuriko HarigayaMasayuki Yamamoto
Mar 16, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takahiro TouganHiroshi Nojima
Feb 13, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·H Sakurai, A Ishihama

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