The Schizosaccharomyces pombe open promoter bubble: mammalian-like arrangement and properties

Journal of Molecular Biology
Wai S ChoiJ D Gralla

Abstract

The fission yeast Schizosaccharomyces pombe is often used as a genetic system to model processes that apply to higher cells. Here S.pombe was used to study promoter DNA opening and transcription initiation by RNA polymerase II. The melted region within the adh promoter is about 20 bp in size and has the start site near its center. This arrangement is similar to that at the AdML promoter but different from that in Saccharomyces cerevisiae. Although expression of human TFIIB shifts the start site to the nearby human position, it does not change the location of the bubble. The start site shift is directed by the C terminus of human TFIIB, in contrast to expectations from S.cerevisiae. The creation of the bubble requires the ATPase motifs of XPB. Overall, the data show that promoter melting and initiation in fission yeast is much more similar to humans than to budding yeast.

References

Jan 1, 1991·Methods in Enzymology·S Sasse-Dwight, J D Gralla
Jan 1, 1987·Annual Review of Genetics·L Guarente
Sep 19, 1980·Science·J CordenP Chambon
Mar 26, 1981·Nature·C Benoist, P Chambon
Jun 25, 1993·Nucleic Acids Research·S L Forsburg
Aug 6, 1993·Science·C Giardina, J T Lis
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Buratowski, H Zhou
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
May 13, 1999·The Journal of Biological Chemistry·N A Hawkes, S G Roberts
Jul 20, 1999·Molecular and Cellular Biology·E Guzmán, J T Lis
Nov 28, 2000·Annual Review of Genetics·T I Lee, R A Young
Jun 25, 2002·Journal of Molecular Biology·Wai S ChoiJay D Gralla
Sep 7, 2002·Molecular and Cellular Biology·Jennifer A FairleyStefan G E Roberts

❮ Previous
Next ❯

Citations

Jun 7, 2005·Nature Structural & Molecular Biology·Yin Chun LinJay D Gralla
Oct 1, 2010·The Journal of Biological Chemistry·Khiem Tran, Jay D Gralla
May 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Henrik SpåhrRoger D Kornberg
Feb 3, 2005·Yeast
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rina FujiwaraKenji Murakami
Feb 10, 2009·Biochemistry·Tapan BiswasOleg V Tsodikov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.