The scs and scs' insulator elements impart a cis requirement on enhancer-promoter interactions

Molecular Cell
J E Krebs, M Dunaway

Abstract

The Xenopus rRNA enhancer activates its cognate promoter when the two elements are placed on opposite rings of dimeric catenanes. Here we show that when scs elements flank either the enhancer or promoter in catenanes, the enhancer cannot activate the promoter on the ring in trans. A series of catenanes containing different permutations of the insulators, enhancer, and promoters shows that when insulators are present, the enhancer is permitted to a activate the promoter only when both elements are on the same piece of DNA with no intervening insulator. These results suggest that insulators have the potential to block enhancer-promoter interactions between chromosomes and between independent topological domains within a chromosome.

References

May 1, 1992·Molecular and Cellular Biology·R Kellum, P Schedl
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W SuH Echols
Sep 1, 1989·Trends in Genetics : TIG·W M StarkD J Sherratt
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D ShoreR L Baldwin
Oct 1, 1996·Molecular and Cellular Biology·J E Krebs, M Dunaway
Jan 1, 1997·Molecular and Cellular Biology·M DunawayH L Yuen
Apr 1, 1997·Current Opinion in Genetics & Development·P K Geyer

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Citations

Apr 28, 1999·Trends in Genetics : TIG·Z Avramova, A Tikhonov
Jun 18, 2002·Current Opinion in Cell Biology·Masaya Oki, Rohinton T Kamakaka
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·W Wei, M D Brennan
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R MorrisC T Wu
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·F Recillas-TargaG Felsenfeld
Mar 7, 2007·Molecular and Cellular Biology·Young Soo YoonKarl Pfeifer
May 14, 1999·Current Opinion in Genetics & Development·A C Bell, G Felsenfeld
May 14, 1999·Current Opinion in Genetics & Development·C T Wu, J R Morris
Aug 26, 1998·Current Biology : CB·R Kellum, S C Elgin
Apr 28, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Geneviève FourelEric Gilson

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