PMID: 2504727Sep 1, 1989Paper

The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast

The Journal of Cell Biology
A Salminen, P Novick

Abstract

SEC15 function is required at a late stage of the yeast secretory pathway. Duplication of the gene encoding the ras-like, GTP-binding protein, Sec4, can suppress the partial loss of function resulting from the sec15-l mutation, but cannot suppress disruption of sec15. Analysis of the SEC15 gene predicts a hydrophilic protein product of 105 kD. Anti-Sec15 antibody recognizes a protein of 116-kD apparent molecular mass which is associated with a microsomal fraction of yeast in a strongly pH dependent fashion. Overproduction of Sec15 protein interferes with the secretory pathway, resulting in the formation of a cluster of secretory vesicles, and a patch of Sec15 protein revealed by immunofluorescence. The sec4-8 and sec2-4l mutations, but not mutations in other SEC genes, prevent formation of the Sec15 protein patch. We propose that Sec15 protein responds to the function of the Sec4 protein to control vesicular traffic.

References

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Citations

Nov 7, 2008·Protoplasma·B Sönnichsen
Aug 1, 1991·Current Opinion in Cell Biology·M G WatersJ E Rothman
Jun 21, 2002·Trends in Cell Biology·Peter Novick, Wei Guo
Oct 1, 1993·Molecular Biology of the Cell·M W CraigheadJ Armstrong
Jan 14, 2005·Molecular Biology of the Cell·Allison ZajacWei Guo
Apr 14, 2006·Molecular Biology of the Cell·Martina MedkovaPeter Novick
Apr 21, 2006·Molecular Biology of the Cell·Johan-Owen De CraenePeter Novick
Oct 5, 2007·Molecular Biology of the Cell·Maria Grazia GiansantiMaurizio Gatti
Oct 10, 2008·Molecular Biology of the Cell·Cornelia KurischkoFrancis C Luca
Oct 16, 2009·Molecular Biology of the Cell·Daniel F MarkgrafChristian Ungermann
Jan 1, 1996·Annual Review of Cell and Developmental Biology·S R Pfeffer
Jun 17, 2006·Annual Review of Neuroscience·Matthew J Kennedy, Michael D Ehlers
Jan 21, 2011·Physiological Reviews·Alex H Hutagalung, Peter J Novick
Dec 15, 2005·BMC Biology·Faith L W LieblDavid E Featherstone
Jul 20, 2002·Seminars in Cell & Developmental Biology·Hao XuWilliam S Trimble
Aug 12, 1998·The Journal of Cell Biology·F P Finger, P Novick
Jul 31, 2013·The Journal of Cell Biology·David ShenPeter Novick
Jan 30, 1991·European Journal of Biochemistry·P J Van HaastertC Erneux
Aug 19, 2008·Trends in Cell Biology·Hao WuPatrick Brennwald
Aug 27, 2014·Traffic·Leah J WatsonPatrick Brennwald

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