PMID: 11607167Mar 15, 1991Paper

The second amino acid of alfalfa mosaic virus coat protein is critical for coat protein-mediated protection

Proceedings of the National Academy of Sciences of the United States of America
N E TumerP R Sanders

Abstract

Transgenic plants expressing the coat protein (CP) of alfalfa mosaic virus (AIMV) are resistant to infection by AIMV. A mutation was introduced into the second amino acid of the cDNA for the CP of AIMV. Three different transgenic tobacco lines expressing the mutant CP and two different transgenic tobacco lines expressing the wild-type CP at similar levels were challenged with AIMV virions and viral RNA. Whereas the lines expressing the wild-type CP were highly resistant to infection by AIMV virions and viral RNA, the lines expressing the mutant CP were susceptible to infection by both. The binding affinity of the mutant and the wild-type CPs for the 3' terminal protein binding site on AIMV RNAs was similar, as determined by electrophoretic mobility shift assay. A mixture of AIMV genomic RNAs 1-3 was infectious on the plants expressing the mutant CP but not on vector control plants or plants expressing the wild-type CP, indicating that the mutant CP can activate the AIMV genomic RNAs for infection. These results demonstrate that the second amino acid of the AIMV CP is critical for protection from AIMV but not for the initial interaction between the AIMV RNA and CP, suggesting that this initial interaction does not play a major r...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 24, 1989·Science·M E BianchiG Paonessa
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Citations

Jan 1, 1994·Archives of Virology·A F HacklandJ A Thomson
Jan 1, 1996·Archives of Virology·M Prins, R Goldbach
Nov 10, 2001·Annual Review of Phytopathology·A CallawayS A Lommel

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