PMID: 9658090Jul 11, 1998Paper

The second extracellular loop of CXCR4 determines its function as a receptor for feline immunodeficiency virus

Journal of Virology
Brian J WillettMargaret J Hosie

Abstract

The feline homolog of the alpha-chemokine receptor CXCR4 has recently been shown to support cell-cell fusion mediated by CXCR4-dependent strains of human immunodeficiency virus (HIV) and strains of feline immunodeficiency virus (FIV) that have been selected for growth in the Crandell feline kidney (CrFK) cell line. In this report we demonstrate that expression of CXCR4 alone is sufficient to render cells from diverse species permissive for fusion with FIV-infected cells, suggesting that CXCR4 is the sole receptor for CrFK-tropic strains of FIV, analogous to CD4-independent strains of HIV-2. To identify the regions of CXCR4 involved in fusion mediated by FIV, we screened panels of chimeric CXCR4 molecules for the ability to support fusion with FIV-infected cells. Human CXCR4 supported fusion more efficiently than feline CXCR4 and feline/human CXCR4 chimeras, suggesting that the second and third extracellular loops of human CXCR4 contain a critical determinant for receptor function. Rat/human CXCR4 chimeras suggested that the second extracellular loop contained the principal determinant for receptor function; however, chimeras constructed between human CXCR2 and CXCR4 revealed that the first and third loops of CXCR4 contribute to...Continue Reading

References

Sep 1, 1990·Journal of Virology·K IkeuchiJ E Groopman
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TatenoJ A Levy
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A V MosesJ A Nelson
May 1, 1995·Veterinary Immunology and Immunopathology·K H SiebelinkM L Bosch
Nov 1, 1993·AIDS Research and Human Retroviruses·C WildT Matthews
Feb 13, 1997·Nature·B J WillettJ A Hoxie
Feb 17, 1997·The Journal of Experimental Medicine·R I ConnorN R Landau
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z LuR W Doms
Dec 31, 1997·The Journal of Experimental Medicine·C B DavisD R Littman
Dec 31, 1997·Science·K A MartinN P Gerard

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Citations

Jul 18, 2001·The Journal of General Virology·T Dragic
Sep 5, 2001·Journal of Virology·F BaribaudR W Doms
Feb 14, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Arnt-Ove Hovden, Maja A Sommerfelt
Sep 13, 2014·Future Virology·Tricia H Burdo, Andrew D Miller
May 20, 1999·Molecular Membrane Biology·B J Willett, M J Hosie
Feb 22, 2008·Veterinary Immunology and Immunopathology·John H ElderYing-Chuan Lin
Feb 21, 2004·Science·Masayuki ShimojimaBrian J Willett

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