The second respiratory chain of Candida parapsilosis: a comprehensive study

Biochimie
M GuerinA Cheyrou

Abstract

The yeast C. parapsilosis CBS7157 is strictly dependent on oxidative metabolism for growth since it lacks a fermentative pathway. It is nevertheless able to grow on high glucose concentrations and also on a glycerol medium supplemented with antimycin A or drugs acting at the level of mitochondrial protein synthesis. Besides its normal respiratory chain C. parapsilosis develops a second electron transfer chain antimycin A-insensitive which allows the oxidation of cytoplasmic NAD(P)H resulting from glycolytic and hexose monophosphate pathways functioning through a route different from the NADH-coenzyme Q oxidoreductase described in S. cerevisiae or from the alternative pathways described in numerous plants and microorganisms. The second respiratory chain of C. parapsilosis involves 2 dehydrogenases specific for NADH and NADPH respectively, which are amytal and mersalyl sensitive and located on the outer face of the inner membrane. Since this antimycin A-insensitive pathway is fully inhibited by myxothiazol, it was hypothesized that electrons are transferred to a quinone pool that is different from the classical coenzyme Q-cytochrome b cycle. Two inhibitory sites were evidenced with myxothiazol, one related to the classical pathwa...Continue Reading

Citations

Aug 1, 1994·Journal of Bioenergetics and Biomembranes·J M JaultA Di Pietro
Nov 7, 2002·Current Genetics·Jozef NosekChoukri Ben Mamoun
Aug 8, 2009·Current Genetics·Jozef NosekLubomir Tomaska
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Aug 23, 1991·Biochimica Et Biophysica Acta·A L Moore, J N Siedow
Jul 25, 2021·Journal of Industrial Microbiology & Biotechnology·Luis B Flores-CoteraOscar Ulises García-Flores

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