PMID: 9421503Feb 28, 1998Paper

The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae

Nucleic Acids Research
K HashimotoAkio Sugino

Abstract

DNA polymerase III (delta) of Saccharomyces cerevisiae is purified as a complex of at least two polypeptides with molecular masses of 125 and 55 kDa as judged by SDS-PAGE. In this paper we determine partial amino acid sequences of the 125 and 55 kDa polypeptides and find that they match parts of the amino acid sequences predicted from the nucleotide sequence of the CDC2 and HYS2 genes respectively. We also show by Western blotting that Hys2 protein co-purifies with DNA polymerase III activity as well as Cdc2 polypeptide. The complex form of DNA polymerase III activity could not be detected in thermosensitive hys2 mutant cell extracts, although another form of DNA polymerase III was found. This form of DNA polymerase III, which could also be detected in wild-type extracts, was not associated with Hys2 protein and was not stimulated by addition of proliferating cell nuclear antigen (PCNA), replication factor A (RF-A) or replication factor C (RF-C). The temperature-sensitive growth phenotype of hys2-1 and hys2-2 mutations could be suppressed by the CDC2 gene on a multicopy plasmid. These data suggest that the 55 kDa polypeptide encoded by the HYS2 gene is one of the subunits of DNA polymerase III complex in S.cerevisiae and is req...Continue Reading

References

Oct 25, 1978·Journal of Molecular Biology·F SangerM Smith
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ChenJ Hurwitz
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H ArakiA Sugino
Sep 21, 1990·Cell·A MorrisonA Sugino
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J H RothmanT H Stevens
Sep 11, 1995·Nucleic Acids Research·K SugimotoK Matsumoto
Sep 1, 1995·Molecular and Cellular Biology·G CullmannB Stillman
Aug 1, 1995·Trends in Biochemical Sciences·A Sugino
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H ArakiA Sugino
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoK Matsumoto
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·S ZuoJ Hurwitz

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Citations

Feb 13, 2001·Trends in Biochemical Sciences·S A MacNeillU Hübscher
Mar 24, 1999·Trends in Biochemical Sciences·M MäkiniemiJ E Syväoja
May 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A SuginoH Araki
Oct 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Narottam AcharyaSatya Prakash
Aug 1, 1998·Nucleic Acids Research·L Aravind, E V Koonin
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kikuo ShimizuAkio Sugino
Oct 13, 2009·Journal of Molecular Biology·Rinku JainAneel K Aggarwal
Jul 19, 2002·The Journal of Biological Chemistry·Kikuo ShimizuAkio Sugino
Sep 25, 2001·The Journal of Biological Chemistry·E JohanssonP M Burgers
May 3, 2002·The Journal of Biological Chemistry·Xiaoqing LuAntero G So
Apr 4, 2013·G3 : Genes - Genomes - Genetics·Bonnie AlverDavid T Kirkpatrick
Jun 6, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jolanda van LeeuwenBrenda J Andrews
Jul 19, 2002·The Journal of Biological Chemistry·Vladimir P BermudezJerard Hurwitz
Apr 10, 2020·Cellular and Molecular Life Sciences : CMLS·Farah KobaisiXavier Gidrol
May 23, 2006·The Journal of Biological Chemistry·Takashi SekiAkio Sugino

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