Infection with the human liver fluke, Opisthorchis viverrini, is a serious public health problem in Thailand, Laos and nearby locations in Southeast Asia. Both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (CCA). To identify parasite proteins critical for liver fluke survival and the etiology of CCA, OFFGEL electrophoresis and multiple reaction monitoring were employed to characterize 300 parasite proteins from the O. viverrini excretory/secretory products and, utilizing selective labeling and sequential solubilization, from the host-exposed tegument. The excretory/secretory included a complex mixture of proteins that have been associated with cancers, including proteases of different mechanistic classes and orthologues of mammalian growth factors and anti-apoptotic proteins. Also identified was a cysteine protease inhibitor which, in other helminth pathogens, induces nitric oxide production by macrophages, and, hence may contribute to malignant transformation of inflamed cells. More than 160 tegumental proteins were identified using sequential solubilization of isolated teguments, and a subset of these was localized to ...Continue Reading
Relationship between intensity of Opisthorchis viverrini infection and hepatobiliary disease detected by ultrasonography
Complete sequence of the Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase gene encoding a major surface antigen
Isolation and sequence of the granulin precursor cDNA from human bone marrow reveals tandem cysteine-rich granulin domains
Opisthorchis viverrini infestation and endogenous nitrosamines as risk factors for cholangiocarcinoma in Thailand.
L-arginine-dependent formation of N-nitrosamines by the cytosol of macrophages activated with lipopolysaccharide and interferon-gamma
Immune responsiveness and parasite-specific antibody levels in human hepatobiliary disease associated with Opisthorchis viverrini infection
Correlation between urinary activity of N-acetyl-beta-D-glucosaminidase (NAG) and albumin excretion rate in type II (non-insulin-dependent) diabetic subjects
Tegument surface membranes of adult Schistosoma mansoni: development of a method for their isolation
Subcellular localization of pyruvate dehydrogenase dihydrolipoamide acetyltransferase in human intrahepatic biliary epithelial cells
Haemonchus contortus: identification of proteases with diverse characteristics in adult worm excretory-secretory products
Biochemical characterization of the extracellular domain of the 75-kilodalton tumor necrosis factor receptor
Cloning, molecular characterization, and functional activity of Schistosoma japonicum glyceraldehyde-3-phosphate dehydrogenase, a putative vaccine candidate against schistosomiasis japonica.
Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis
Detection of endogenous biotin-containing proteins in bone and cartilage cells with streptavidin systems
Onchocerca volvulus: microfilariae secrete elastinolytic and males nonelastinolytic matrix-degrading serine and metalloproteases
Relationship between parasite-specific antibody responses and intensity of Opisthorchis viverrini infection in hamsters
The nature and prospects for gut membrane proteins as vaccine candidates for Haemonchus contortus and other ruminant trichostrongyloids
Prevalence of Opisthorchis viverrini infection and incidence of cholangiocarcinoma in Khon Kaen, Northeast Thailand
The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host-parasite interactions
Increased cell proliferation of mouse fibroblast NIH-3T3 in vitro induced by excretory/secretory product(s) from Opisthorchis viverrini
An analysis of the calcium-binding protein 1 of Fasciola gigantica with a comparison to its homologs in the phylum Platyhelminthes
Ancylostoma caninum MTP-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration
The tegument surface membranes of the human blood parasite Schistosoma mansoni: a proteomic analysis after differential extraction
Identification of novel proteases and immunomodulators in the secretions of schistosome cercariae that facilitate host entry.
Proteomic analysis of the tegument and excretory-secretory products of adult Schistosoma bovis worms
Thioredoxin reductase 1 deficiency reverses tumor phenotype and tumorigenicity of lung carcinoma cells.
Identification and characterization of the second cysteine protease inhibitor of Clonorchis sinensis (CsStefin-2)
Platyhelminth Venom Allergen-Like (VAL) proteins: revealing structural diversity, class-specific features and biological associations across the phylum.
A deep exploration of the transcriptome and "excretory/secretory" proteome of adult Fascioloides magna.
Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins
Unlocking the transcriptomes of two carcinogenic parasites, Clonorchis sinensis and Opisthorchis viverrini.
Differential activation of diverse glutathione transferases of Clonorchis sinensis in response to the host bile and oxidative stressors
Coming out of the shell: building the molecular infrastructure for research on parasite-harbouring snails
Insights into SCP/TAPS proteins of liver flukes based on large-scale bioinformatic analyses of sequence datasets.
Identification and characterization of paramyosin from cyst wall of metacercariae implicated protective efficacy against Clonorchis sinensis infection.
Proteomic identification of plasma protein tyrosine phosphatase alpha and fibronectin associated with liver fluke, Opisthorchis viverrini, infection.
Suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, Opisthorchis viverrini
Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract
Molecular and biochemical characterizations of three fructose-1,6-bisphosphate aldolases from Clonorchis sinensis
Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products
Proteomic identification of potential Clonorchis sinensis excretory/secretory products capable of binding and activating human hepatic stellate cells
Proteomic characterization of the internalization of Opisthorchis viverrini excretory/secretory products in human cells
Genomics of worms, with an emphasis on Opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes
Phylogenetic characterization of Clonorchis sinensis proteins homologous to the sigma-class glutathione transferase and their differential expression profiles
Chromosomes and karyotype analysis of a liver fluke, Opisthorchis viverrini, by scanning electron microscopy
Cloning, expression, and characterization of a novel Opisthorchis viverrini calcium-binding EF-hand protein
Secreted Opisthorchis viverrini glutathione S-transferase regulates cell proliferation through AKT and ERK pathways in cholangiocarcinoma
Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans
Progress on the transcriptomics of carcinogenic liver flukes of humans--unique biological and biotechnological prospects
Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype
Inflammatory response to liver fluke Opisthorchis viverrini in mice depends on host master coregulator MTA1, a marker for parasite-induced cholangiocarcinoma in humans.
Adult Opisthorchis felineus major protein fractions deduced from transcripts: comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
Identification and functional characterization of CsStefin-1, a cysteine protease inhibitor of Clonorchis sinensis
Fructose-bisphosphate aldolase and enolase from Echinococcus granulosus: genes, expression patterns and protein interactions of two potential moonlighting proteins
Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity
Suppression of Ov-grn-1 encoding granulin of Opisthorchis viverrini inhibits proliferation of biliary epithelial cells
Similarity of a 16.5kDa tegumental protein of the human liver fluke Opisthorchis viverrini to nematode cytoplasmic motility protein
Systems biology studies of adult paragonimus lung flukes facilitate the identification of immunodominant parasite antigens
Carcinogenic Parasite Secretes Growth Factor That Accelerates Wound Healing and Potentially Promotes Neoplasia
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