The secreted lipoprotein, MPT83, of Mycobacterium tuberculosis is recognized during human tuberculosis and stimulates protective immunity in mice.

PloS One
Fan F KaoW J Britton

Abstract

The long-term control of tuberculosis (TB) will require the development of more effective anti-TB vaccines, as the only licensed vaccine, Mycobacterium bovis bacille Calmette-Guérin (BCG), has limited protective efficacy against infectious pulmonary TB. Subunit vaccines have an improved safety profile over live, attenuated vaccines, such as BCG, and may be used in immuno-compromised individuals. MPT83 (Rv2873) is a secreted mycobacterial lipoprotein expressed on the surface of Mycobacterium tuberculosis. In this study, we examined whether recombinant MPT83 is recognized during human and murine M. tuberculosis infection. We assessed the immunogenicity and protective efficacy of MPT83 as a protein vaccine, with monophosphyl lipid A (MPLA) in dimethyl-dioctadecyl ammonium bromide (DDA) as adjuvant, or as a DNA vaccine in C57BL/6 mice and mapped the T cell epitopes with peptide scanning. We demonstrated that rMPT83 was recognised by strong proliferative and Interferon (IFN)-γ-secreting T cell responses in peripheral blood mononuclear cells (PBMC) from patients with active TB, but not from healthy, tuberculin skin test-negative control subjects. MPT83 also stimulated strong IFN-γ T cell responses during experimental murine M. tuberc...Continue Reading

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Citations

Apr 5, 2014·Acta Crystallographica. Section D, Biological Crystallography·Jinal ShuklaB Gopal
Dec 18, 2013·BMC Microbiology·Olabisi Oluwabukola CokerPrasit Palittapongarnpim
Aug 26, 2014·Expert Opinion on Biological Therapy·Nicolas BruffaertsMarta Romano
Mar 4, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Yih-Yuan ChenHorng-Yunn Dou
Sep 17, 2015·Veterinary Immunology and Immunopathology·Chuang MengXinan Jiao
Oct 19, 2012·Virulence·Marina A ForrelladFabiana Bigi
Feb 18, 2012·The Journal of Allergy and Clinical Immunology·Norbert MeyerCezmi Akdis
May 17, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lin WangBaoxue Ge
Mar 7, 2018·Advanced Healthcare Materials·Leon Chien-Wei LinChe-Ming Jack Hu
Apr 6, 2018·Scientific Reports·Emily J Strong, Nicholas P West

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Methods Mentioned

BETA
ELISA
flow cytometry
scintillation spectroscopy

Software Mentioned

SYFPEITHI
IEDB
Kodak IM
GraphPad Prism

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