The secretion of Mullerian inhibiting substance by cultured isolated Sertoli cells of the neonatal calf

The American Journal of Anatomy
J M Price

Abstract

Sertoli cells have been insolated from the newborn calf testis using a combination of mechanical and enzymatic disruption. Testicular fragments, previously chopped into 1-mm pieces, are digested in an enzyme mixture consisting of hyaluronidase, collagenase, trypsin and DNAse, followed by a second digestion in trypsin and DNAse. Isolation of the resulting cellular fractions by sedimentation with unit gravity produces an aliquot of Sertoli cells which is over 95% pure when examined by light and electron microscopy. Cultures of these cells grow rapidly and produce Mullerian Inhibiting Substance as evidenced by their ability to cause the involution of the Mullerian duct of the female fetal rat when co-cultured in an organ-culture assay system.

References

Jun 1, 1977·Journal of Pediatric Surgery·P K DonahoeW H Hendren
Mar 1, 1977·Biology of Reproduction·P K DonahoeW H Hendren
Jul 1, 1977·The American Journal of Anatomy·J M PriceW H Hendren
Aug 1, 1977·The Journal of Surgical Research·P K DonahoeW H Hendren
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A R BellvéD A O'Brien
Mar 1, 1976·The American Journal of Anatomy·H Merchant-Larios
Oct 1, 1976·Biology of Reproduction·P K DonahoeW H Hendren
Feb 1, 1971·Biology of Reproduction·A Steinberger, E Steinberger
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY

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Citations

Feb 18, 2009·In Vitro Cellular & Developmental Biology. Animal·M KorujiA J Arfaee
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R M DePhilip, A L Kierszenbaum
Mar 1, 1993·Molecular Reproduction and Development·K M KelleyJ Ilan
Jul 1, 1986·The Journal of Urology·M LaQuagliaP K Donahoe
Sep 1, 1986·Clinical Endocrinology·N Josso

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