The secretions of oviduct epithelial cells increase the equine in vitro fertilization rate: are osteopontin, atrial natriuretic peptide A and oviductin involved?

Reproductive Biology and Endocrinology : RB&E
Sylvie MugnierGhylène Goudet

Abstract

Oviduct epithelial cells (OEC) co-culture promotes in vitro fertilization (IVF) in human, bovine and porcine species, but no data are available from equine species. Yet, despite numerous attempts, equine IVF rates remain low. Our first aim was to verify a beneficial effect of the OEC on equine IVF. In mammals, oviductal proteins have been shown to interact with gametes and play a role in fertilization. Thus, our second aim was to identify the proteins involved in fertilization in the horse. In the first experiment, we co-incubated fresh equine spermatozoa treated with calcium ionophore and in vitro matured equine oocytes with or without porcine OEC. We showed that the presence of OEC increases the IVF rates. In the subsequent experiments, we co-incubated equine gametes with OEC and we showed that the IVF rates were not significantly different between 1) gametes co-incubated with equine vs porcine OEC, 2) intact cumulus-oocyte complexes vs denuded oocytes, 3) OEC previously stimulated with human Chorionic Gonadotropin, Luteinizing Hormone and/or oestradiol vs non stimulated OEC, 4) in vivo vs in vitro matured oocytes. In order to identify the proteins responsible for the positive effect of OEC, we first searched for the presence...Continue Reading

References

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Citations

Dec 1, 2011·Reproduction, Fertility, and Development·Ghylène Goudet
Dec 19, 2012·Reproduction, Fertility, and Development·Monika Nowak-Imialek, Heiner Niemann
Jun 30, 2010·Molecular Human Reproduction·Manuel AvilésPilar Coy
Jun 1, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Barbara AmbruosiGhylène Goudet
Jan 23, 2016·Theriogenology·Salvatore DesantisGiovanni Michele Lacalandra
Nov 1, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Bart LeemansAnn Van Soom
Sep 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Sergio GhersevichCarlos Zumoffen
Feb 6, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Bart LeemansBart M Gadella
Aug 14, 2019·Zygote : the Biology of Gametes and Early Embryos·Anise AsaadiMiel Hostens
Apr 15, 2020·Animals : an Open Access Journal From MDPI·Katiuska SatuéPietro Medica
Apr 4, 2021·International Journal of Molecular Sciences·Carla Moros-NicolásMª José Izquierdo-Rico
Dec 7, 2021·Frontiers in Cell and Developmental Biology·Paula Piccolo MaitanHeiko Henning

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Methods Mentioned

BETA
FCS
biosensor
chip
electrophoresis
surface plasmon resonance

Software Mentioned

Genewise
Ensembl
MEGA4
Tblastn

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