The Secretome Derived From 3D-Cultured Umbilical Cord Tissue MSCs Counteracts Manifestations Typifying Rheumatoid Arthritis

Frontiers in Immunology
Joana P MirandaJorge M Santos

Abstract

Rheumatoid arthritis (RA) is an autoimmune disorder whose treatment is mostly restricted to pain and symptom management and to the delay of joint destruction. Mesenchymal stem/stromal cells from the umbilical cord tissue (UC-MSCs) have previously been proven to be immunomodulatory and more efficient than bone marrow-derived MSCs in causing remission of local and systemic arthritic manifestations in vivo. Given the paracrine nature of UC-MSC activity, their application as active substances can be replaced by their secretome, thus avoiding allogeneic rejection and safety issues related to unwanted grafting. In this work, we aimed at demonstrating the viability of applying the 3D-primed UC-MSC secretome for the amelioration of arthritic signs. A proteomic analysis was performed to both, media conditioned by UC-MSC monolayer (CM2D) and 3D cultures (CM3D). The analysis of relevant trophic factors confirmed secretome profiles with very significant differences in terms of therapeutic potential. Whereas, CM3D was characterised by a prevailing expression of anti-inflammatory cytokines such as IL-10 and LIF, along with trophic factors involved in different mechanisms leading to tissue regeneration, such as PDGF-BB, FGF-2, I-309, SCF, and...Continue Reading

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Citations

Jun 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lu LiuXiaofeng Chen
Jul 22, 2019·Human Genetics·Taylor McNeelyIsabel Beerman
Jun 20, 2020·Stem Cell Reviews and Reports·Maria BousnakiPetros Koidis
Jul 25, 2019·International Journal of Molecular Sciences·Jaime FreitasMaria Inês Almeida
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Siddharth ShanbhagKamal Mustafa
Oct 8, 2020·American Journal of Physiology. Cell Physiology·Hualing SunVictor J Dzau
Jan 9, 2021·Stem Cell Research & Therapy·Mesude BicerDarius Widera
Mar 20, 2021·Stem Cell Research & Therapy·Alexander MarkovAli Hassanzadeh
Apr 16, 2021·Scientific Reports·Sophia Bou-GhannamTeruo Okano
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Rebecca M HarmanGerlinde R Van de Walle
Sep 24, 2021·Stem Cell Reviews and Reports·M BousnakiP Koidis

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Methods Mentioned

BETA
protein assay
Assay
flow cytometry
light microscopy

Software Mentioned

GraphPad Prism
Flow
Kaluza
Motic Images
[UNK] Pro

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