The secretome of apoptotic human peripheral blood mononuclear cells attenuates secondary damage following spinal cord injury in rats

Experimental Neurology
Thomas HaiderHendrik Jan Ankersmit

Abstract

After spinal cord injury (SCI), secondary damage caused by oxidative stress, inflammation, and ischemia leads to neurological deterioration. In recent years, therapeutic approaches to trauma have focused on modulating this secondary cascade. There is increasing evidence that the success of cell-based SCI therapy is due mainly to secreted factors rather than to cell implantation per se. This study investigated peripheral blood mononuclear cells as a source of factors for secretome- (MNC-secretome-) based therapy. Specifically, we investigated whether MNC-secretome had therapeutic effects in a rat SCI contusion model and its possible underlying mechanisms. Rats treated with MNC-secretome showed substantially improved functional recovery, attenuated cavity formation, and reduced acute axonal injury compared to control animals. Histological evaluation revealed higher vascular density in the spinal cords of treated animals. Immunohistochemistry showed that MNC-secretome treatment increased the recruitment of CD68(+) cells with concomitant reduction of oxidative stress as reflected by lower expression of inducible nitric oxide synthase. Notably, MNC-secretome showed angiogenic properties ex vivo in aortic rings and spinal cord tissue...Continue Reading

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Citations

Jul 21, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Maria GaluppoEmanuela Mazzon
Oct 28, 2016·Apoptosis : an International Journal on Programmed Cell Death·Lucian BeerHendrik Jan Ankersmit
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Sep 25, 2019·International Journal of Molecular Sciences·Milada ChudickovaSarka Kubinova
Aug 12, 2016·European Journal of Clinical Investigation·Mohammad Mahdi KasiriHendrik Jan Ankersmit
Nov 19, 2020·International Journal of Molecular Sciences·Layla PanahipourReinhard Gruber
Feb 4, 2021·Bioengineering & Translational Medicine·Stefan HackerHendrik J Ankersmit
Mar 27, 2021·Molecular Therapy. Methods & Clinical Development·Maria LaggnerHendrik Jan Ankersmit

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