The secretory granule protein syncollin localizes to HL-60 cells and neutrophils

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Jan-Philipp BachMichael Schrader

Abstract

The secretory granule protein syncollin was first identified in the exocrine pancreas where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In this study we provide first morphological and biochemical evidence that, in addition to its pancreatic localization, syncollin is also present in neutrophilic granulocytes of rat and human origin. By immunohistological studies, syncollin was detected in neutrophilic granulocytes of the spleen. Furthermore, syncollin is expressed by the promyelocytic HL-60 cells, where it is stored in azurophilic granules and in a vesicular compartment. These findings were confirmed by fractionation experiments and immunoelectron microscopy. Treatment with a phorbol ester triggered the release of syncollin indicating that in HL-60 cells it is a secretory protein that can be mobilized upon stimulation. A putative role for syncollin in host defense is discussed.

References

Jan 1, 1996·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·A H Gitter
May 20, 1998·European Journal of Cell Biology·H DartschH F Kern
Feb 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·S Tan, S C Hooi
Feb 7, 2001·The Journal of Biological Chemistry·S J AnJ M Edwardson
Jul 14, 2001·Archives of Oral Biology·A ImaiH Shimomura
Sep 18, 2002·The Journal of Membrane Biology·N A GeisseJ M Edwardson
Jan 17, 2003·Molecular Biology of the Cell·Charles GuerraPeter Satir
Nov 14, 2003·Microbes and Infection·Mikkel Faurschou, Niels Borregaard
Oct 7, 2004·The Biochemical Journal·Barbara WäsleJ Michael Edwardson
Oct 12, 2004·Journal of Proteome Research·Mads GrønborgAkhilesh Pandey
Nov 4, 2004·American Journal of Physiology. Renal Physiology·Sebastian BachmannSatish Kumar
Jun 30, 2005·Molecular & Cellular Proteomics : MCP·George LominadzeKenneth R McLeish

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Citations

Jun 21, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Q SunH Q Liu
Oct 27, 2010·Expert Review of Proteomics·María Gómez-LázaroMichael Schrader
Jun 12, 2012·Experimental Cell Research·Cornelia RinnMichael Schrader
May 21, 2011·FEBS Letters·Johan W M HeemskerkAlastair W Poole
Aug 17, 2010·Journal of Proteome Research·Heike BortaMichael Schrader
Jun 22, 2021·Cellular Microbiology·Rosie A WatersJ Michael Edwardson

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Datasets Mentioned

BETA
BC039541

Methods Mentioned

BETA
FCS
fluorescence
density gradient centrifugation
ELISA
electrophoresis
PCR
dot-blot
dot blots
electron microscopy
PMA

Software Mentioned

Pcbas
Adobe Photoshop
Excel

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