The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo

International Journal of Molecular Sciences
Francesco De FrancescoGiuseppe A Ferraro

Abstract

Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes. The results showed that in the middle-high density (MHD) layer there was a peak concentration of ASCs, compared to another layer obtained after centrifugation. Research carried out on patients under treatment for soft tissue regeneration using cells obtained from MHD layer selection will be fundamental in comparative analysis. These studies will lead to an adequate standardization of outcomes, provided that treatment is perfo...Continue Reading

References

Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jul 2, 2003·Cells, Tissues, Organs·Daniel A De UgarteMarc H Hedrick
Sep 11, 2008·Tissue Engineering. Part C, Methods·Francesco D'AndreaGianpaolo Papaccio
Dec 6, 2008·Facial Plastic Surgery Clinics of North America·Yadranko Ducic
Jun 6, 2009·Aesthetic Plastic Surgery·Yves Gérard Illouz, Aris Sterodimas
Aug 15, 2009·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Alexandra Condé-GreenIvo Pitanguy
Oct 24, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ludovic ZimmerlinAlbert D Donnenberg
Nov 17, 2009·The Breast Journal·Kotaro YoshimuraKiyonori Harii
Nov 12, 2010·Aesthetic Plastic Surgery·Giuseppe A FerraroFrancesco D'Andrea
May 19, 2011·Annals of Plastic Surgery·Joseph P HunstadBill G Kortesis
Nov 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ludovic ZimmerlinAlbert D Donnenberg
Mar 29, 2013·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Marc GuibertNatacha Kadlub
Sep 17, 2013·The Journal of Craniofacial Surgery·Marco KlingerValeriano Vinci
Feb 1, 2014·Surgical Innovation·Despoina Kakagia, Norbert Pallua
Jul 24, 2014·Plastic and Reconstructive Surgery·Harunosuke KatoKotaro Yoshimura
Oct 8, 2014·Plastic and Reconstructive Surgery. Global Open·Ondrej MestakAndrej Sukop
Jan 17, 2015·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Riaz A AghaDennis P Orgill
Feb 13, 2015·Frontiers in Surgery·Lipi ShuklaRamin Shayan
Feb 20, 2015·Annals of Plastic Surgery·Albert LoskenFrancesco M Egro
May 9, 2015·Tissue Engineering. Part B, Reviews·Francesco De FrancescoGiuseppe Andrea Ferraro
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Francesca CecchinatoRyo Jimbo
Jun 4, 2015·Expert Opinion on Biological Therapy·Yoshiaki ShingyochiHiroshi Mizuno
Jun 29, 2015·Clinics in Plastic Surgery·Sydney R Coleman, Evan B Katzel
Jun 29, 2015·Clinics in Plastic Surgery·Tomasz R KosowskiRoger K Khouri
Dec 29, 2015·Plastic and Reconstructive Surgery·Lihong QiuChenggang Yi
Feb 4, 2016·Stem Cells International·Bommie Florence Seo, Sung-No Jung
Feb 16, 2016·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Roger K Khouri, Thomas M Biggs
Mar 12, 2016·Journal of Visualized Experiments : JoVE·Valeria PurpuraMichele Riccio
Jul 15, 2016·Cancer Biology & Therapy·Francesco De FrancescoUmberto Cillo

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Citations

May 1, 2020·International Journal of Molecular Sciences·Elena Dai PrèAndrea Sbarbati
May 22, 2018·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Yao YaoFeng Lu
Oct 2, 2019·Medicina·Cristian Ilie DrochioiVictor Vlad Costan
Nov 20, 2019·Clinics in Plastic Surgery·Shaoheng XiongLee L Q Pu
Sep 15, 2021·The Journal of Craniofacial Surgery·Zhe CaoXiao-Qin Liang

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Methods Mentioned

BETA
PCR
FACS
Profiler
imaging techniques

Software Mentioned

Excel
SPSS
PCR Array Data Analysis Templates

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