The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase

The Journal of Cell Biology
Kazuki KumadaTetsuo Noda

Abstract

Separase is an evolutionarily conserved protease that is essential for chromosome segregation and cleaves cohesin Scc1/Rad21, which joins the sister chromatids together. Although mammalian separase also functions in chromosome segregation, our understanding of this process in mammals is still incomplete. We generated separase knockout mice, reporting an essential function for mammalian separase. Separase-deficient mouse embryonic fibroblasts exhibited severely restrained increases in cell number, polyploid chromosomes, and amplified centrosomes. Chromosome spreads demonstrated that multiple chromosomes connected to a centromeric region. Live observation demonstrated that the chromosomes of separase-deficient cells condensed, but failed to segregate, although subsequent cytokinesis and chromosome decondensation proceeded normally. These results establish that mammalian separase is essential for the separation of centromeres, but not of the arm regions of chromosomes. Other cell cycle events, such as mitotic exit, DNA replication, and centrosome duplication appear to occur normally. We also demonstrated that heterozygous separase-deficient cells exhibited severely restrained increases in cell number with apparently normal mitosis...Continue Reading

References

Aug 25, 1981·Journal of Molecular Biology·N Sternberg, D Hamilton
Jan 29, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M Yanagida
Sep 28, 2000·Biochemical and Biophysical Research Communications·I ImotoJ Inazawa
Jan 9, 2001·Nature Cell Biology·A Amon
May 24, 2001·Cell·P V JallepalliC Lengauer
Dec 19, 2001·Cell·O StemmannM W Kirschner
Aug 27, 2002·Current Biology : CB·Irene WaizeneggerJan-Michael Peters
Jun 7, 2005·Genes & Development·Ana Losada, Tatsuya Hirano
Sep 3, 2005·Nature Cell Biology·Maura PapiPrasad V Jallepalli

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Citations

Oct 6, 2006·Chromosoma·W RensM A Ferguson-Smith
Aug 27, 2013·Nature·Antonio TedeschiJan-Michael Peters
Sep 2, 2006·Nature Cell Biology·Marie-Emilie Terret, Prasad V Jallepalli
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nenggang ZhangDebananda Pati
Jan 11, 2007·Genes & Development·Jennifer L ShepardLeonard I Zon
Nov 6, 2009·Annual Review of Genetics·Kim Nasmyth, Christian H Haering
Apr 9, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rene MeyerDebananda Pati
May 21, 2010·Cell Division·Alexander V Strunnikov
Mar 29, 2007·PloS One·Laura A Díaz-MartínezDuncan J Clarke
Apr 30, 2014·Nature Cell Biology·Hyun-Ja Nam, Jan M van Deursen
Jun 6, 2009·Analytical Biochemistry·Dipanjan BasuDebananda Pati
Sep 8, 2015·Current Opinion in Cell Biology·Vijay Pratap Singh, Jennifer L Gerton
May 14, 2008·Biochimica Et Biophysica Acta·Ana Losada
Jan 1, 2008·Molecular and Cellular Endocrinology·Jérôme Artus, Michel Cohen-Tannoudji
Jun 19, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Vincent Guacci
Apr 22, 2008·Journal of Cellular Biochemistry·Amanda Gonçalves Dos Santos SilvaSabine Mai
Nov 30, 2011·Physiologia Plantarum·Panagiotis N Moschou, Peter V Bozhkov
May 9, 2012·EMBO Reports·Andrew J Holland, Don W Cleveland
Oct 19, 2006·Current Opinion in Cell Biology·Beth A A Weaver, Don W Cleveland
Feb 18, 2011·Nucleus·Martin Kucej, Hui Zou
Jul 28, 2013·Current Biology : CB·Gabriela CabralAlexander Dammermann
Feb 11, 2010·Current Biology : CB·Sandra López-Avilés, Frank Uhlmann
Dec 27, 2006·Cancer Cell·Rocío SotilloRobert Benezra
Sep 18, 2009·Developmental Cell·Meng-Fu Bryan TsouPrasad V Jallepalli
Apr 10, 2010·Fertility and Sterility·Stephanie BeallJames Segars
Apr 24, 2007·Journal of Cellular Physiology·H N PembertonC J McCabe
Dec 15, 2016·Journal of Cellular Biochemistry·Ravinder Kumar
Feb 9, 2017·Biological Reviews of the Cambridge Philosophical Society·Nenggang Zhang, Debananda Pati
Nov 23, 2017·Nucleic Acids Research·Francesco CuccoAntonio Musio
Dec 30, 2017·The Journal of Cell Biology·Jadranka Loncarek, Mónica Bettencourt-Dias
Jul 25, 2006·Nature·Meng-Fu Bryan Tsou, Tim Stearns
May 2, 2014·The EMBO Journal·Susanne HellmuthOlaf Stemmann
Jul 3, 2007·The Journal of Biological Chemistry·Andrew J HollandStephen S Taylor
Oct 16, 2016·Cold Spring Harbor Perspectives in Medicine·Magali De Koninck, Ana Losada
Nov 3, 2007·The Journal of Biological Chemistry·Dominik BoosOlaf Stemmann

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Methods Mentioned

BETA
PCR
dissection
FACS
flow cytometry
electrophoresis
confocal microscopy

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