The selective utilization of substrates in vivo by the phosphatidylethanolamine and phosphatidylcholine biosynthetic enzymes Ept1p and Cpt1p in yeast

FEBS Letters
H A BoumannAnton I P M de Kroon

Abstract

In yeast, the aminoalcohol phosphotransferases Ept1p and Cpt1p catalyze the final steps in the CDP-ethanolamine and CDP-choline routes leading to phosphatidylethanolamine (PE) and phosphatidylcholine (PC), respectively. To determine how these enzymes contribute to the molecular species profiles of PE and PC in vivo, wild-type, cpt1Delta, and ept1Delta cells were pulse labeled with deuterated ethanolamine and choline. Analysis of newly synthesized PE and PC using electrospray ionization tandem mass spectrometry revealed that PE and PC produced by Ept1p and Cpt1p have different species compositions, demonstrating that the enzymes consume distinct sets of diacylglycerol species in vivo. Using the characteristic phospholipid species profiles produced by Ept1p and Cpt1p as molecular fingerprints, it was also shown that in vivo CDP-monomethylethanolamine is preferentially used as substrate by Ept1p, whereas CDP-dimethylethanolamine and CDP-propanolamine are converted by Cpt1p.

References

Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·B BrüggerW D Lehmann
Nov 25, 1997·Biochimica Et Biophysica Acta·M I Kanipes, S A Henry
Sep 11, 2002·Molecular Biology of the Cell·Annette L HenneberryChristopher R McMaster
Aug 2, 2003·Current Opinion in Cell Biology·Vytas A Bankaitis, Andrew J Morris
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea

❮ Previous
Next ❯

Citations

Apr 25, 2012·PloS One·Christian KloseKai Simons
Aug 22, 2014·Molecular Biology of the Cell·Aline Xavier da Silveira Dos SantosHoward Riezman
Jan 15, 2011·The FEBS Journal·Mauro Serricchio, Peter Bütikofer
Jun 17, 2014·The FEBS Journal·Meriyem AktasFranz Narberhaus
May 30, 2006·Biochimica Et Biophysica Acta·J Pedro Fernández-Murray, Christopher R McMaster
May 2, 2013·Progress in Lipid Research·Anton I P M de KroonCedric H De Smet
Jul 13, 2006·Methods : a Companion to Methods in Enzymology·Alan N Hunt, Anthony D Postle
Jan 29, 2016·Lipid Insights·Mike F RenneAnton I P M de Kroon
Feb 3, 2005·Yeast
Oct 4, 2006·The Journal of Biological Chemistry·Wayne R Riekhof, Dennis R Voelker
Oct 1, 2020·Mass Spectrometry Reviews·Changfeng HuXianlin Han
Apr 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anthony D Postle, Alan N Hunt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.