The selectivity of Vibrio cholerae H-NOX for gaseous ligands follows the "sliding scale rule" hypothesis. Ligand interactions with both ferrous and ferric Vc H-NOX

Biochemistry
Gang WuAh-lim Tsai

Abstract

Vc H-NOX (or VCA0720) is an H-NOX (heme-nitric oxide and oxygen binding) protein from facultative aerobic bacterium Vibrio cholerae. It shares significant sequence homology with soluble guanylyl cyclase (sGC), a NO sensor protein commonly found in animals. Similar to sGC, Vc H-NOX binds strongly to NO and CO with affinities of 0.27 nM and 0.77 μM, respectively, but weakly to O2. When positioned on a "sliding scale" plot [Tsai, A.-l., et al. (2012) Biochemistry 51, 172-186], the line connecting log K(D)(NO) and log K(D)(CO) of Vc H-NOX can almost be superimposed with that of Ns H-NOX. Therefore, the measured affinities and kinetic parameters of gaseous ligands to Vc H-NOX provide more evidence to validate the "sliding scale rule" hypothesis. Like sGC, Vc H-NOX binds NO in multiple steps, forming first a six-coordinate heme-NO complex at a rate of 1.1 × 10(9) M(-1) s(-1), and then converts to a five-coordinate heme-NO complex at a rate that is also dependent on NO concentration. Although the formation of oxyferrous Vc H-NOX cannot be detected at a normal atmospheric oxygen level, ferrous Vc H-NOX is oxidized to the ferric form at a rate of 0.06 s(-1) when mixed with O2. Ferric Vc H-NOX exists as a mixture of high- and low-spin st...Continue Reading

References

Mar 18, 1976·Biochimica Et Biophysica Acta·T NozawaM Hatano
Jun 1, 1985·Biochemical Society Transactions·G Palmer
Sep 28, 1981·FEBS Letters·K R CarterG Palmer
Jan 1, 1995·Annual Review of Physiology·O W Griffith, D J Stuehr
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y XiaJ L Zweier
Mar 1, 1996·Journal of Magnetic Resonance. Series B·C GalliG W Brudvig
Nov 14, 1997·The American Journal of Physiology·S D SavkovicG Hecht
Aug 26, 1998·The Journal of Biological Chemistry·Y XiaJ L Zweier
Dec 3, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rinku Jain, Michael K Chan
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patricia PellicenaJohn Kuriyan
Oct 9, 2004·Science·Pierre NiocheC S Raman
Aug 30, 2005·Current Opinion in Chemical Biology·Elizabeth M Boon, Michael A Marletta
Jan 12, 2007·The EMBO Journal·Xiaolei MaFocco van den Akker
Jun 25, 2010·Biochemistry·Ah-Lim TsaiJohn S Olson
Mar 13, 2012·Annual Review of Biochemistry·Emily R Derbyshire, Michael A Marletta
Apr 13, 2012·Antioxidants & Redox Signaling·Aisha FarhanaAdrie J C Steyn
Jun 7, 2012·MBio·Danilo G MorielRino Rappuoli
Mar 15, 2013·Biochimica Et Biophysica Acta·Ursula LieblMarten H Vos

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Citations

Sep 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mark A HerzikMichael A Marletta
Aug 30, 2017·Antioxidants & Redox Signaling·Dominique E WilliamsElizabeth Boon
Oct 16, 2018·Chembiochem : a European Journal of Chemical Biology·Yirui Guo, Michael A Marletta
Oct 3, 2017·Nature Chemical Biology·Yirui GuoMichael A Marletta
Jul 1, 2016·The Journal of Biological Chemistry·Roma MukhopadyayErik T Yukl
Apr 20, 2021·Trends in Plant Science·Aloysius WongChristoph Gehring
Aug 1, 2018·ACS Chemical Biology·Sajjad HossainElizabeth M Boon
Nov 7, 2018·Biochemistry·Yirui GuoMichael A Marletta

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