PMID: 2349Jan 1, 1976

The self-association of adenosine-5'-triphosphate studied by circular dichroism at low ionic strengths

Biophysical Chemistry
T J Gilligan, G Schwarz


The self-association of adenosine-5'-triphosphate (ATP) was studied as a function of pH, additional counterions, concentration and temperature. Circular dichroism measurements were employed as a measure of the base-stacking. The self-association of ATP is pH dependent with the protonation of the adenine ring helping stabilize the association. Highly charged counterions alter this aggregation. At pH 2.8 and 20 degrees C, a dimerization constant of 88 M-1 is obtained, while an isodesmic model leads to an equilibrium constant of 158 M-1. With increasing pH, the association constants decrease. At pH 2.8 there is a very strong temperature dependence of the CD amplitude. These results indicate the existence of additional electrostatic stabilization for the stacking of the adenine rings. At acidic pHs, models are proposed to explain this high degree of stability and a calculation of the approximate electrostatic contribution to the aggregation shows it to be of the proper magnitude.


Feb 1, 1975·Biophysical Chemistry·M P Heyn, R Bretz
Feb 15, 1973·The Journal of Physical Chemistry·M G Marenchic, J M Sturtevant
Mar 20, 1974·Journal of the American Chemical Society·Y F LamG Kotowycz
Feb 1, 1974·FEBS Letters·T A GlassmanT J Swift
Apr 24, 1972·European Journal of Biochemistry·D Pörschke, F Eggers
Jan 24, 1970·Nature·O KennardL R Di Sanseverino
Feb 14, 1968·Journal of the American Chemical Society·M P SchweizerD P Hollis
Mar 21, 1967·Biochemical and Biophysical Research Communications·G P Rossetti, K E Van Holde
May 1, 1974·Biophysical Chemistry·W E FergusonG H Barlow


Oct 13, 2006·Biophysical Chemistry·Matthias Bastian, Helmut Sigel
Jul 11, 1980·Nucleic Acids Research·A A Maevsky, B I Sukhorukov
Jul 17, 1978·European Journal of Biochemistry·P R Mitchell, Helmut Sigel
Jan 4, 1988·European Journal of Biochemistry·R Tribolet, Helmut Sigel
Oct 10, 2003·Nucleosides, Nucleotides & Nucleic Acids·Yael Richter, Bilha Fischer
Jun 28, 1977·Biochemistry·G Schwarz, T J Gilligan
Dec 23, 2016·Molecular and Cellular Biochemistry·Akhil VarshneyPramod K Yadava

Related Concepts

Adenosine Triphosphate, Chromium Ammonium Salt
Circular Dichroism, Vibrational
Hydrogen-Ion Concentration
Spectrophotometry, Ultraviolet

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