PMID: 7536753Feb 1, 1995Paper

The self-renewal process of murine hemopoietic stem cells supported by interleukin-3 and the synergistic factors and the probability of its occurrence

Nihon Ika Daigaku zasshi
K Kaneko, K Ikebuchi

Abstract

By use of the in vitro murine blast cell colony (Bl) assay system, Bl-constituting cells supported by interleukin-3 (IL-3), IL-3 + interleukin-6 (IL-6), IL-3 + granulocyte colony-stimulating factor (G-CSF) and IL-3 + interleukin-1 (IL-1) were replated and the frequencies of secondary (2nd) granulocyte/erythrocyte/macrophage/megakaryocyte (GEMM) colony and Bl progenitors were examined. According to the statistical method reported by Nakahata et al, the p values for the hemopoietic stem cells to self-renew were calculated and all cytokine groups produced similar p values ranging between 0.576 and 0.596. Further studies using IL-3-supported and IL-3 + G-CSF-supported Bl showed that the 2nd Bl progenitors could be produced even when there were more than 150 primary Bl-constituting cells per colony in the case of IL-3 + G-CSF, but no 2nd Bl progenitors were found in the case of IL-3. Their appearance was limited within the smaller primary Bl when supported by IL-3. Again there was a difference in the net product number of 2nd Bl progenitors, that is, the addition of G-CSF to the primary culture could produce around double the number of 2nd Bl progenitors. These data led us to hypothesize that synergistic factors could not modify the...Continue Reading

Citations

Aug 7, 2009·Stem Cells and Development·Pedro Bordeaux-RegoDaniella Pereira Crosara-Alberto

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