The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein

Gene
Y W LeeE W Nester

Abstract

The virulence (vir) genes of Agrobacterium tumefaciens are induced by low-molecular-weight phenolic compounds and monosaccharides through a two-component regulatory system consisting of the VirA and VirG proteins. Although it is clear that the monosaccharides require binding to a periplasmic binding protein before they can interact with the sensor VirA protein, it is not certain whether the phenolic compounds also interact with a binding protein or directly interact with the sensor protein. To shed light on this question, we tested the vir-inducing abilities of several different phenolic compounds using two wild-type strains of A. tumefaciens, KU12 and A6. We found that several compounds such as 4-hydroxyacetophone and p-coumaric acid induced the vir of KU12, but not A6. On the other hand, acetosyringone and several other phenolic compounds induced the vir of A6, but not KU12. By transferring different Ti plasmids into isogenic chromosomal backgrounds, we showed that the phenolic sensing determinant is associated with the Ti plasmid. Subcloning of the Ti plasmid indicated that the virA locus determines which phenolic compounds can function as vir inducers. These results suggest that VirA directly senses the phenolic compounds f...Continue Reading

References

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Citations

Jun 15, 1999·Trends in Microbiology·J L Rakeman, S I Miller
Mar 25, 2011·Nature Chemistry·Mohammad R SeyedsayamdostJon Clardy
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
May 20, 2006·Annual Review of Cell and Developmental Biology·Colleen A McCullen, Andrew N Binns
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·Aseneth Herrera-MartínezBeatriz Xoconostle-Cázares
Dec 10, 2015·Plant Molecular Biology·Eva Czarnecka-VernerWilliam B Gurley
Aug 11, 2010·Molecular Plant Pathology·Amita BhattacharyaVitaly Citovsky
Sep 26, 2003·Molecular Microbiology·Martin W BaderSamuel I Miller
Oct 11, 2011·Microbial Pathogenesis·Florencia IanninoNora Iñón de Iannino
Aug 29, 2013·Methods : a Companion to Methods in Enzymology·Jeffrey M VargasonJesse W Wilson
Aug 12, 2018·The Plant Journal : for Cell and Molecular Biology·Mohammad Reza RoushanGerard Paul H van Heusden
Oct 20, 2017·The Arabidopsis Book·Hau-Hsuan HwangErh-Min Lai
Aug 28, 2015·Chembiochem : a European Journal of Chemical Biology·Fang FangDavid G Lynn

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