PMID: 8972850Dec 1, 1996Paper

The sensitivity of human fibroblasts to N-acetoxy-2-acetylaminofluorene is determined by the extent of transcription-coupled repair, and/or their capability to counteract RNA synthesis inhibition

Nucleic Acids Research
M F van OosterwijkA A van Zeeland

Abstract

Nucleotide excision repair (NER) mechanism is the major pathway responsible for the removal of a large variety of bulky lesions from the genome. Two different NER subpathways have been identified, i.e. the transcription-coupled and the global genome repair pathways. For DNA-damage induced by ultraviolet light both transcription-coupled repair and global genome repair are essential to confer resistance to cytotoxic effects. To gain further insight into the contribution of NER subpathways in the repair of bulky lesions and in their prevention of biological effects we measured the rate of repair of dG-C8-AF in active and inactive genes in normal human cells, XP-C cells (only transcription-coupled repair) and XP-A cells (completely NER-deficient) exposed to NA-AAF. XP-C cells are only slightly more sensitive to NA-AAF than normal cells and, like normal cells, they are able to recover RNA synthesis repressed by the treatment. In contrast, XP-A cells are sensitive to NA-AAF and unable to recover from RNA synthesis inhibition. Repair of dG-C8-AF in the active ADA gene proceeds in a biphasic way and without strand specificity, with a subclass of lesions quickly repaired during the first 8 h after treatment. Repair in the inactive 754 g...Continue Reading

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Citations

Jul 31, 2003·Life Sciences·Hilde E van GijsselJohn H N Meerman
Aug 1, 1998·Mutation Research·H VrielingL H Mullenders
Dec 5, 1998·Mutation Research·L H Mullenders
Oct 20, 1999·Mutation Research·N YeG P Holmquist
Nov 22, 2008·Nature Reviews. Molecular Cell Biology·Philip C Hanawalt, Graciela Spivak
Dec 16, 2005·Molecular and Cellular Biology·Guochun Jiang, Aziz Sancar
Apr 2, 2013·Mechanisms of Ageing and Development·Bruce C McKay, Miguel A Cabrita
May 6, 2008·DNA Repair·Thierry Nouspikel
Jun 14, 2006·Mutation Research·Susan W P WijnhovenHarry van Steeg
Aug 28, 2002·Journal of the European Academy of Dermatology and Venereology : JEADV·S Dubaele, J M Egly
Feb 9, 2006·Chemical Reviews·Ludovic C J Gillet, Orlando D Schärer

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