PMID: 12787678Jun 6, 2003Paper

The sensitivity of lipid domains to small perturbations demonstrated by the effect of Triton

Journal of Molecular Biology
Heiko HeerklotzJoachim Seelig

Abstract

The hypothesis of lipid rafts describes functional domains in biological membranes. It is often assumed that rafts form by spontaneous de-mixing of certain lipids and that they can be isolated as detergent-resistant membrane particles (DRMs) using the detergent Triton X-100 (TX). Here, we present a model that describes the process of domain formation in membranes in the presence and in the absence of TX. We measure the interactions between TX and an equimolar mixture of sphingomyelin (SM), cholesterol (Cho), and 1-palmitoyl-2-oleoyl-3-sn-glycero-phosphatidylcholine (POPC) (1:1:1, mol) by means of isothermal titration calorimetry. Comparison with pure POPC membranes reveals a very unfavorable interaction between TX and SM/Cho, which causes a substantial tendency to segregate these molecules into separate, DRM-like (SM-rich) and fluid (TX-rich), domains. If rafts are indeed formed by spontaneous de-mixing of PC and SM/Cho, they must be very sensitive, and perturbations caused by techniques used to study rafts could lead to misleading results. If, however, rafts are much more stable than PC-SM-Cho domains, there must be an unknown raft stabilizer. Subtle changes of such a promoter could serve to modulate raft function.

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Citations

May 20, 2009·The Journal of Membrane Biology·Nathan C RockwellJeremy Thorner
Apr 20, 2004·Advanced Drug Delivery Reviews·Shefali Sabharanjak, Satyajit Mayor
Dec 17, 2008·Quarterly Reviews of Biophysics·Heiko Heerklotz
Sep 15, 2007·Nature Protocols·Daniel Lingwood, Kai Simons
Oct 20, 2007·Nature Reviews. Immunology·Antonella Viola, Neetu Gupta
Apr 21, 2006·Nature Reviews. Molecular Cell Biology·John F Hancock
Aug 30, 2008·The Journal of Biological Chemistry·Corey M JohnsonWilliam Rodgers
Nov 13, 2003·Genome Biology·Anthony I Magee, Ingela Parmryd
Dec 17, 2008·Journal of Lipid Research·Soazig Le LayKatharina Gaus
Jul 7, 2009·Journal of Lipid Research·Yu Zi Zheng, Leonard J Foster
Mar 25, 2011·Journal of Pharmacy & Bioallied Sciences·Antonio RaudinoMartina Pannuzzo
Mar 16, 2013·The Journal of Biological Chemistry·Meenakshi SharmaAbraham Kovoor
May 19, 2010·Progress in Lipid Research·Peter J Quinn
Oct 28, 2008·Biochimica Et Biophysica Acta·Félix M GoñiJenifer L Thewalt
Oct 18, 2008·Journal of Proteomics·Yu Zi Zheng, Leonard J Foster
Nov 18, 2015·The Journal of General Physiology·Radda RusinovaOlaf S Andersen
Apr 21, 2005·Cellular Microbiology·Frank Lafont, F Gisou van der Goot
Nov 25, 2003·EMBO Reports·Chiara ZurzoloSatyajit Mayor
Jan 24, 2009·Annals of the New York Academy of Sciences·Kendra L FurberJens R Coorssen
Nov 8, 2005·Biophysical Journal·Stefanie Y NishimuraW E Moerner
Sep 11, 2007·Biophysical Journal·Jihong TongThomas J McIntosh
Oct 16, 2007·Biophysical Journal·Ashley E GarnerNigel M Hooper
Apr 26, 2005·Biophysical Journal·Cristina ArnulphiAna Jonas
Oct 4, 2006·Free Radical Biology & Medicine·Anna Wisniewska, Witold K Subczynski
Jul 6, 2005·Trends in Biochemical Sciences·Dov LichtenbergHeiko Heerklotz
Mar 20, 2004·Traffic·J Bernd Helms, Chiara Zurzolo
Mar 20, 2004·Traffic·Satyajit Mayor, Madan Rao
Mar 25, 2009·The Biochemical Journal·Mercedes Ingelmo-TorresAlbert Pol

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