PMID: 9435274Jan 22, 1998Paper

The sensitivity of the human Kv1.3 (hKv1.3) lymphocyte K+ channel to regulation by PKA and PKC is partially lost in HEK 293 host cells

The Journal of Membrane Biology
J MartelM D Payet

Abstract

cDNA encoding the full-length hKv1.3 lymphocyte channel and a C-terminal truncated (delta 459-523) form that lacks the putative PKA Ser468 phosphorylation site were stably transfected in human embryonic kidney (HEK) 293 cells. Immunostaining of the transfected cells revealed a distribution at the plasma membrane that was uniform in the case of the full-length channel whereas clustering was observed in the case of the truncated channel. Some straining within the cell cytoplasm was found in both instances, suggesting an active process of biosynthesis. Analyses of the K+ current by the patch-clamp technique in the whole cell configuration showed that depolarizing steps to 40 mV from a holding potential (HP) of -80 mV elicited an outward current of 2 to 10 nA. The current threshold was positive to -40 mV and the current amplitude increased in a voltage-dependent manner. The parameters of activation were -5.7 and -9.9 mV (slope factor) and -35 mV (half activation, V0.5) in the case of the full-length and truncated channels, respectively. The characteristics of the inactivation were 14.2 and 24.6 mV (slope factor) and -17.3 and -39.0 mV (V0.5) for the full-length and truncated channels, respectively. The activation time constant of t...Continue Reading

Citations

Apr 12, 2003·Neuropharmacology·M WinklhoferS Saaler-Reinhardt
Jan 26, 2008·The Journal of Biological Chemistry·Rubén VicenteAntonio Felipe
Mar 2, 2012·American Journal of Physiology. Cell Physiology·Zerrin KurasLaura Conforti
Dec 25, 2004·Physiological Reviews·Steven C HebertWenhui Wang
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shigeru ShibataRichard P Lifton
Mar 13, 2013·The Journal of Physiology·Kristal TuckerDebra Ann Fadool
Dec 19, 2009·American Journal of Physiology. Cell Physiology·Elyssa D BurgJason X-J Yuan
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Jan 31, 2006·Microsurgery·Andrea FerenczErzsbet Roth
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Apr 15, 2017·Frontiers in Pharmacology·Alicia de la CruzTeresa Gonzalez
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rubén VicenteAntonio Felipe
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Aug 5, 2000·American Journal of Physiology. Renal Physiology·S C SansomD A Hall
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·F PotetI Baró

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