The Separation and the Characterization of Long Chain Fatty Acids and Their Derivatives by Reversed Phase High Performance Liquid Chromatography

Critical Reviews in Analytical Chemistry
Phyllis R BrownJoseph Turcotte

Abstract

The potential for the application of chromatography to the analysis of fatty acids was first realized by A. J. James and A. J. P. Martin in 1952(1). These two noted scientists successfully separated the iso- and ante-isomers of short chain free fatty acids by gas liquid chromatography (GLC). Even today, 35 years later, the method of choice for characterization of fatty acids is capillary gas chromatography with a mass spectrometer as a detector. However, high performance liquid chromatography (HPLC) is now becoming competitive in the separation of fatty acids, especially on the preparative scale.

References

Aug 6, 1975·Journal of Chromatography· Aitzetmüller
Sep 1, 1975·Journal of Chromatographic Science·M J Cooper, M W Anders
Jul 1, 1976·Analytical Chemistry·N E Hoffman, J C Liao
Jun 12, 1987·Journal of Chromatography·R J HamiltonP A Sewell
Feb 28, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·H MiwaT Nishida
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Oct 1, 1982·Journal of Chromatographic Science·A G BailieD W Hill
Jan 1, 1982·Progress in Lipid Research·K Aitzetmüller
Oct 14, 1988·Science·M J GordonR N Zare

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Citations

Jul 22, 2010·International Journal of Toxicology·Lillian C BeckerF Alan Andersen
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·G Gutnikov
Jul 30, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tara CarpenterMitchell E Johnson

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