The sequence and function of the recA gene and its protein in Pseudomonas aeruginosa PAO

Molecular & General Genetics : MGG
Y Sano, M Kageyama

Abstract

The recA gene of Pseudomonas aeruginosa has been isolated and its nucleotide sequence has been determined. The coding region of the recA gene has 1038 bp specifying 346 amino acids. The recA protein of P. aeruginosa showed a striking homology with that of Escherichia coli except for the carboxy-terminal region both at the nucleotide and amino acid level. The recA+-carrying plasmids restored the UV sensitivity and recombination ability of several rec mutants of P. aeruginosa. The precise location of the recA gene on the chromosome was deduced from the analysis of R' plasmids.

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Related Concepts

Pseudomonas aeruginosa (antigen)
Rec A Recombinases
Alkalescens-Dispar Group
Amino Acids, I.V. solution additive
Recombination, Genetic
RAD51 wt Allele
RAD51 gene
Pseudomonas aeruginosa
Genes, Bacterial
Nucleotides

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