The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea

International Journal of Molecular Sciences
Junjie YanBaogui Xie

Abstract

As the first defence for cells to counteract the toxicity of active oxygen, superoxide dismutase (SOD) plays an important role in the response of living organisms to stress and cell differentiation. One extracellular Cu-ZnSOD (ecCu-ZnSOD), and two MnSODs, were identified based on the Volvariella volvacea genome sequence. All three genes have complicated alternative splicing modes during transcription; only when the fourth intron is retained can the Vv_Cu-Znsod1 gene be translated into a protein sequence with SOD functional domains. The expression levels of the three sod genes in the pilei are higher than in the stipe. The Vv_Cu-Znsod1 and the Vv_Mnsod2 are co-expressed in different developmental stages of the fruiting body, with the highest level of expression in the pilei of the egg stage, and they show a significant, positive correlation with the efficiency of karyogamy, indicating the potential role of these two genes during karyogamy. The expression of the ecCu-Znsod and two Vv_Mnsod genes showed a significant up-regulated when treated by cold stress for one hour; however, the lack of the intracellular Cu-ZnSOD encoding gene (icCu-Znsod) and the special locus of the ecCu-Znsod gene initiation codon suggested a possible reas...Continue Reading

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Citations

Nov 2, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Jun-Jie YanBao-Gui Xie
May 22, 2018·Journal of Forensic Nursing·Meredith ScannellNichole Boyer

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Datasets Mentioned

BETA
GME8899
GME1978
GME5987
KP747455
GME5781
ACCC52633

Methods Mentioned

BETA
environmental stress
glycosylation
PCR
Assay

Software Mentioned

Perl scripts
ORF Finder
MEGA5
BLASTP
MEGA
SOAPdenovo assembler
GeneDoc
Excel
ZOOM
SPSS Statistics

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