PMID: 11333022May 3, 2001Paper

The sequence complementarity between HIV-1 5' splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA

RNA
S KammlerHeiner Schaal

Abstract

HIV-1 env expression from certain subgenomic vectors requires the viral regulatory protein Rev, its target sequence RRE, and a 5' splice site upstream of the env open reading frame. To determine the role of this splice site in the 5'-splice-site-dependent Rev-mediated env gene expression, we have subjected the HIV-1 5' splice site, SD4, to a mutational analysis and have analyzed the effect of those mutations on env expression. The results demonstrate that the overall strength of hydrogen bonding between the 5' splice site, SD4, and the free 5' end of the U1 snRNA correlates with env expression efficiency, as long as env expression is suboptimal, and that a continuous stretch of 14 hydrogen bonds can lead to full env expression, as a result of stabilizing the pre-mRNA. The U1 snRNA-mediated stabilization is independent of functional splicing, as a mismatch in position +1 of the 5' splice site that led to loss of detectable amounts of spliced transcripts did not preclude stabilization and expression of the unspliced env mRNA, provided that Rev enables its nuclear export. The nucleotides capable of participating in U1 snRNA:pre-mRNA interaction include positions -3 to +8 of the 5' splice site and all 11 nt constituting the single-...Continue Reading

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Citations

Dec 5, 2003·Biochimica Et Biophysica Acta·Rebeca Martínez-ContrerasJesús Valdés
Jan 28, 2006·AIDS Research and Human Retroviruses·Saowakon Paca-UccaralertkunTun-Hou Lee
May 24, 2012·Genome Biology and Evolution·Rodger B VoelkerJ Andrew Berglund
Jul 26, 2002·Nucleic Acids Research·Tracy FarrerAlan M Zahler
Nov 25, 2003·Nucleic Acids Research·Marcel FreundHeiner Schaal
Jan 18, 2006·Nucleic Acids Research·Tilman HeiseHeiner Schaal
Dec 8, 2009·Nucleic Acids Research·Joseph A JablonskiMassimo Caputi
Oct 12, 2010·Journal of Virology·Dibyakanti MandalC Martin Stoltzfus
Dec 21, 2012·Journal of Virology·Marek WideraHeiner Schaal
Mar 21, 2006·Retrovirology·Alan W CochraneAndrew J Mouland
Dec 6, 2006·Retrovirology·Susanne KammlerHeiner Schaal
Aug 26, 2014·Nucleic Acids Research·Steffen ErkelenzHeiner Schaal
Jun 18, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Meike HassStephan Günther
May 3, 2007·The Journal of Gene Medicine·Maria B AsparuhovaDaniel Schümperli
Jan 20, 2010·The FEBS Journal·Jamal TaziChristiane Branlant
Oct 2, 2012·Journal of Immunological Methods·Eugenia Corrales-AguilarHartmut Hengel
Apr 19, 2015·Retrovirology·Steffen ErkelenzHeiner Schaal
Sep 10, 2014·Antioxidants & Redox Signaling·Sabrina FarrokhHeiner Schaal
Feb 5, 2008·Molecular Cell·Christian Kroun DamgaardJørgen Kjems
Oct 11, 2016·Scientific Reports·Ann BaeyensBruno Verhasselt

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