PMID: 8995696Feb 1, 1997Paper

The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA

Journal of Virology
S FukushiK Katayama

Abstract

Translation of hepatitis C virus (HCV) RNA is initiated by internal entry of ribosomes into the 5' noncoding region (NCR). This process depends on genomic elements within the 5' NCR called the internal ribosome entry site (IRES) and may involve host factors. The alpha-branch structure (nucleotides 47 to 67) of the HCV IRES is considered a cis-acting element critical for translation initiation because it is indispensable for translation in vitro (S. Fukushi, K. Katayama, C. Kurihara, N. Ishiyama, F. B. Hoshino, T. Ando, and A. Oya, Biochem. Biophys. Res. Commun. 199:425-432, 1994). In order to further characterize the function of the alpha-branch, we determined whether sequence exchange within the alpha-branch had any effect on translation initiation. An in vitro translation study revealed that the stem sequences of this region played an important role in efficient IRES function. In addition to several HeLa cell proteins, which had a binding affinity for the 5' NCR, a novel 25-kDa protein that specifically interacted with the HCV IRES was discovered. The binding affinity of the 25-kDa protein for the 5' NCR was correlated with the efficiency of translation initiation of HCV RNA, indicating a critical role for the 25-kDa protein ...Continue Reading

References

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Citations

May 21, 1999·The Journal of Biological Chemistry·N Mamiya, H J Worman
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Stephen A Locarnini
Oct 7, 2010·The Korean Journal of Hepatology·Jae Young Jang, Raymond T Chung
Feb 1, 2002·FEBS Letters·Katerina I KalliampakouPenelope Mavromara
Dec 16, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A VallejoE Lissen
Dec 21, 2000·The Plant Journal : for Cell and Molecular Biology·P UrwinP M Gilmartin
Nov 4, 2000·Journal of Gastroenterology and Hepatology·T KandaH Saisho
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M KrugerF Wong-Staal
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
May 2, 2001·The Journal of Biological Chemistry·S FukushiK Katayama
Mar 12, 1999·Journal of Virology·M H Chen, T K Frey
Jan 27, 1999·Biochemical and Biophysical Research Communications·A VarakliotiP Mavromara
Sep 25, 1999·Journal of Molecular Biology·J S KieftJ A Doudna
Jul 23, 2002·Journal of Virology·Honey V Reddi, Howard L Lipton
Apr 13, 2005·Virology·Angela M DomitrovichAleem Siddiqui

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