The sequence of the human genome

Science
J C VenterX Zhu

Abstract

A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. The 14.8-billion bp DNA sequence was generated over 9 months from 27,271,853 high-quality sequence reads (5.11-fold coverage of the genome) from both ends of plasmid clones made from the DNA of five individuals. Two assembly strategies-a whole-genome assembly and a regional chromosome assembly-were used, each combining sequence data from Celera and the publicly funded genome effort. The public data were shredded into 550-bp segments to create a 2.9-fold coverage of those genome regions that had been sequenced, without including biases inherent in the cloning and assembly procedure used by the publicly funded group. This brought the effective coverage in the assemblies to eightfold, reducing the number and size of gaps in the final assembly over what would be obtained with 5.11-fold coverage. The two assembly strategies yielded very similar results that largely agree with independent mapping data. The assemblies effectively cover the euchromatic regions of the human chromosomes. More than 90% of the genome is in scaffold assemblies of 100,000 bp or more, and 25% of the genom...Continue Reading

References

Sep 19, 2007·Archives of Surgery·Catherine D DeAngelisBoris Pasche
Mar 20, 2008·JAMA : the Journal of the American Medical Association·Phil B FontanarosaCatherine D DeAngelis
May 29, 2008·JAMA : the Journal of the American Medical Association·Linda M DongUlrike Peters
Sep 25, 2001·The Journal of Pathology·K J Hillan, P Quirke
Aug 22, 2001·Biopolymers·M Bina, E Crowely
Jun 27, 2007·Chembiochem : a European Journal of Chemical Biology·Dustin J MalyJonathan A Ellman
Aug 31, 2002·Angewandte Chemie·K ZouH Bayley
Oct 10, 2001·Angewandte Chemie·Günther WessBirgitt Sickenberger
Dec 18, 2001·European Journal of Immunology·J LiippoO Lassila
Sep 5, 2002·Electrophoresis·Wei-Lung TsengHuan-Tsung Chang
Sep 26, 2002·Electrophoresis·Andy J TomlinsonRoman M Chicz
Dec 22, 2004·American Journal of Human Biology : the Official Journal of the Human Biology Council·Carol M Worthman, Jennifer Kuzara
Jan 10, 2002·Annals of Neurology·Katrina Gwinn-Hardy, Matt Farrer
Aug 24, 2001·The Anatomical Record·G W Weber
Apr 16, 2002·Arthritis and Rheumatism·Ethan P GrantJose-Carlos Gutierrez-Ramos
Apr 16, 2002·Arthritis and Rheumatism·William H RobinsonPaul J Utz
Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P A Wade
Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Derek E Wildman
May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C I SmithM Vihinen
Jun 1, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B NalP Ferrier
Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Sachdev S SidhuKurt Deshayes
Jul 17, 2008·Comparative and Functional Genomics·David HendersonYang Da
Jul 17, 2008·Comparative and Functional Genomics·A CottageG Elgar
Jan 5, 2002·Cytometry·Gerrit LosJames K Breaux
Jan 20, 2005·Electrophoresis·Robert J MeagherGary W Slater
Apr 30, 2002·Genes, Chromosomes & Cancer·Kurt A KrummelDavid I Smith
Apr 30, 2002·International Journal of Cancer. Journal International Du Cancer·Kari Hemminki, Xinjun Li
Feb 14, 2002·Journal of Cellular Biochemistry. Supplement·R A Goodnow
Jul 13, 2002·Journal of Computational Chemistry·Yuan-Ping PangFranklyn G. Prendergast
Feb 9, 2002·Journal of Clinical Laboratory Analysis·Peter E BarkerCatherine D O'Connell
Jan 8, 2003·The Journal of Gene Medicine·Joseph P SarseroPanos A Ioannou
Dec 18, 2001·Journal of Molecular Recognition : JMR·H KohlerT Kieber-Emmons
Jan 31, 2003·Journal of Molecular Recognition : JMR·Dobrin Nedelkov, Randall W Nelson
Sep 5, 2002·Journal of Surgical Oncology·Ya-Guang LiuRuo-Pan Huang
Apr 1, 2003·Mass Spectrometry Reviews·Cameron E Forde, Sandra L McCutchen-Maloney

Citations

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 24, 1977·Nature·F SangerM Smith
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·A Martin-GallardoM R Wallace
Aug 1, 1992·Genomics·F LarsenH Prydz
Feb 13, 1992·Nature·M D AdamsJ C Venter
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·M A JensenE Y Chen
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K Meyer-SieglerM A Sirover
Nov 1, 1989·Genomics·R L Sinsheimer
May 1, 1989·Trends in Genetics : TIG·W A Bickmore, A T Sumner
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J D GocayneJ C Venter
Apr 1, 1986·Analytical Biochemistry·E C StraussL E Hood
Sep 21, 1987·Journal of Theoretical Biology·J Maynard Smith
Jul 20, 1987·Journal of Molecular Biology·M Gardiner-Garden, M Frommer
May 27, 1983·Science·N Chaudhari, W E Hahn
Aug 25, 1983·Nucleic Acids Research·R J Milner, J G Sutcliffe
Dec 25, 1982·Journal of Molecular Biology·F SangerG B Petersen
Mar 25, 1981·Journal of Molecular Biology·T F Smith, M S Waterman
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F Antequera, A P Bird
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jun 1, 1995·Current Opinion in Genetics & Development·S H Cross, A P Bird
Oct 20, 1995·Science·C M FraserJ C Venter
Apr 1, 1995·Trends in Neurosciences·J T Littleton, H J Bellen
Jan 11, 1995·Nucleic Acids Research·A F Smit, A D Riggs
Feb 4, 1994·Journal of Molecular Biology·A KroghD Haussler
Dec 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P Garcia-MeunierF Bonhomme
Aug 11, 1993·Nucleic Acids Research·O WhiteC Fields
Aug 1, 1993·Nature Genetics·M H PolymeropoulosC R Merril
May 30, 1996·Nature·J C VenterL Hood
Jan 1, 1996·Methods in Enzymology·E C UberbacherR J Mural
Jan 1, 1996·Annual Review of Biochemistry·D A GoodenoughD L Paul
Oct 25, 1996·Science·A GoffeauS G Oliver

Related Concepts

Vertebrates
Immune System
Chromosome Banding
DNA, Junk
Whole-Genome Shotgun Sequencing
Exons
Genome
Tract
Selfish DNA
Genome Assembly Sequence

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Related Papers

Nature
E S LanderInternational Human Genome Sequencing Consortium
Nature
J D McPhersonInternational Human Genome Mapping Consortium
Nature
International Human Genome Sequencing Consortium
© 2020 Meta ULC. All rights reserved