The sequential cleavage of membrane anchored pro-EGF requires a membrane serine protease other than kallikrein in rat kidney

Regulatory Peptides
Sylvain M Le GallCatherine Dreux

Abstract

Epidermal growth factor (EGF) is present in kidney membranes as an integral type I precursor protein, enzymatically processed to release immunoreactive materials in urine or incubation medium. The aim of this work was the elucidation of both the anchor of the serine protease activity that processes pro-EGF, and the determination of the steps of the enzymatic processing. Quantification of EGF containing molecules by RIA following gel filtration analysis demonstrated that the membrane precursor is first shed from the kidney membrane principally into a 170-kDa soluble precursor. This entire ectodomain is further processed into a 70-kDa precursor and finally into the mature 5.9 kDa urinary EGF. These species correspond to the ones found in urines. Both shedding and maturation events are clearly realized by membrane anchored serine protease activity, which remains active in detergent. By use of wild-type and knockout mice urines, we found that tissue kallikrein (TK) was not involved in the regulation of this processing.

References

Oct 1, 1991·Molecular and Cellular Endocrinology·J D PenschowJ P Coghlan
Apr 1, 1991·Clinical Biochemistry·B OrsiniC Surrenti
Jul 15, 1988·The Biochemical Journal·R J MacDonaldE G Erdös
May 16, 1988·Biochemical and Biophysical Research Communications·J SarosiekB L Slomiany
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·P Cuatrecasas
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J M TaylorW M Mitchell
Jun 15, 1984·Biochemical and Biophysical Research Communications·J ChaoH S Margolius
Jan 1, 1993·Annual Review of Biochemistry·J Massagué, A Pandiella
Jan 15, 1997·The Biochemical Journal·N M HooperA J Turner
Dec 31, 1997·American Journal of Reproductive Immunology : AJRI·H JiangH Zhang
Mar 10, 1999·The American Journal of Physiology·H MaréchalP Mauduit
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·J DongH S Wiley
May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L G KömüvesE Fodor
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·P MenetonF Alhenc-Gelas
Aug 30, 2003·The Journal of Biological Chemistry·Sylvain M Le GallPhilippe Mauduit

❮ Previous
Next ❯

Citations

Feb 26, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laurianne Van LandeghemMichel Neunlist
Apr 16, 2011·EMBO Reports·Colin AdrainMatthew Freeman
Mar 14, 2012·Clinical Science·Anke L LamerisJoost G J Hoenderop
Jul 11, 2006·The Journal of Biological Chemistry·Carmen M WarrenRalf Landgraf
Aug 30, 2014·World Journal of Gastroenterology : WJG·Yan-Bo Yu, Yan-Qing Li
Jan 7, 2011·The Journal of Biological Chemistry·Estelle RousseletNabil G Seidah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.