The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

PloS One
Edi FeroXhelil Koleci

Abstract

Brucellosis is a ubiquitous zoonotic disease globally. It is endemic among bovines, sheep, and goats in Albania. The national control and eradication programs for brucellosis has been applied on sheep and goat farms as well as large dairy cattle farms, i.e., those with more than ten milking cows. The current study aims at estimating the herd and average individual animal prevalence of brucellosis in the national beef cattle herds, the missing information that was essential to propose the most appropriate control measures for this sub-population. Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA), and Enzyme-Linked Immunosorbent Assay (ELISA) were used as serological tests and classical bacteriology for isolation. Results were also used to investigate the difference in sensitivity between the assays used. In total, 655 animals from 38 beef cattle herds from six southern districts of Albania were sampled. Sera were tested using RBT, FPA, and ELISA. Fifteen positive cows and a bull from eight high-prevalence positive herds were slaughtered, and particular tissue samples were collected for bacteriology. The overall herd seroprevalence in the tested beef cattle population was 55%, while the overall average within-herd pre...Continue Reading

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Citations

Mar 29, 2021·Journal of Veterinary Science·Alisha Wehdnesday Bernardo ReyesSuk Kim

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Methods Mentioned

BETA
Enzyme-Linked Immunosorbent Assay
Fluorescence
fluorescence polarization assay
blood collection
ELISA
biotyping
PCR

Software Mentioned

Sentry®
cELISA
Excel
Pak

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