The SET protein promotes androgen production in testicular Leydig cells

Andrology
Bei ZhangYugui Cui

Abstract

Approximately 40% of middle-aged men exhibit symptoms of late-onset hypogonadism (LOH). However, the mechanism of androgen deficiency is still currently unclear. As shown in our previous studies, the SET protein is expressed in testicular Leydig cells and ovarian granule cells. This study was designed to investigate the effect of the SET protein on androgen production in Leydig cells. The AdCMV/SET and AdH1siRNA/SET adenoviruses were individually transduced into a cultured mouse Leydig cell line (mLTC-1) with or without human chorionic gonadotropin (HCG) stimulation in vitro. The primary mouse Leydig cells were used to confirm the main data from mLTC-1 cells. The SET protein was expressed in the cytoplasm and nucleus of mLTC-1 cells. Testosterone production was significantly increased in mLTC-1 cells overexpressing the SET protein compared with the control group (p < 0.05), whereas testosterone production was significantly decreased in the SET knockdown mLTC-1 cells (p < 0.05). Consistent with the testosterone levels, the expression levels of the steroidogenic acute regulatory (StAR) and cytochrome P450c17α-hydroxylase (CYP17a1) mRNAs and proteins synchronously changed according to the expression level of the SET protein. Inter...Continue Reading

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Methods Mentioned

BETA
protein assay
fluorescence microscopy
transfection
gene knockout
GTPases
confocal microscopy

Software Mentioned

zeiss lsm browser

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