The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world

Proceedings of the National Academy of Sciences of the United States of America
Marie-Pierre EgloffBruno Canard


The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-A resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.


Nov 1, 1995·Trends in Biochemical Sciences·L Holm, C Sander
Dec 1, 1995·Proteins·J Janin, F Rodier
Aug 15, 1997·Structure·C A OrengoJ M Thornton
Dec 16, 1998·Trends in Biochemical Sciences·S F Altschul, E V Koonin
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·A A VaguineS J Wodak
May 13, 1999·Bioinformatics·P GouetF Métoz
Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Feb 22, 2002·Acta Crystallographica. Section D, Biological Crystallography·Zbigniew DauterEleanor Dodson
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas C Terwilliger
Dec 31, 2002·Current Opinion in Structural Biology·Vickery Arcus
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
May 6, 2003·Science·Marco A MarraRachel L Roper
Aug 15, 2003·The Journal of General Virology·Volker ThielJohn Ziebuhr
Aug 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Valérie CampanacciChristian Cambillau
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon

❮ Previous
Next ❯


Aug 8, 2007·Journal of Structural and Functional Genomics·Mark BartlamZihe Rao
May 12, 2009·Nature Reviews. Microbiology·Stanley Perlman, Jason Netland
Oct 18, 2005·Nature Structural & Molecular Biology·Yujia ZhaiZihe Rao
Nov 17, 2005·Journal of Neurovirology·Samson S Y Wong, K Y Yuen
Oct 31, 2009·Nucleic Acids Research·Aartjan J W te VelthuisEric J Snijder
Apr 4, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Stéfano RicagnoMarie Pierre Egloff
May 2, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Wei WangZihe Rao
Jun 3, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yanlin MaXuemei Li
Mar 12, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Claire DebarnotBruno Canard
Oct 16, 2007·Clinical Microbiology Reviews·Vincent C C ChengKwok Yung Yuen
Aug 25, 2006·Journal of Virology·Stanley G SawickiStuart G Siddell
Dec 17, 2009·Journal of Virology·Marne C HagemeijerCornelis A M de Haan
Sep 22, 2006·Journal of Virology·Heike SchützeJohn Ziebuhr
Mar 28, 2008·Journal of Virology·Benjamin W NeumanPeter Kuhn
Mar 30, 2007·Journal of Virology·Eric F DonaldsonRalph S Baric
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Karen L Maxwell, Lori Frappier
May 23, 2006·Annual Review of Microbiology·Luis EnjuanesSonia Zuñiga
Aug 24, 2005·Genetic Vaccines and Therapy·Ali AziziFrancisco Diaz-Mitoma
Jan 5, 2005·PLoS Biology·Michael P RobertsonWilliam G Scott
Dec 13, 2005·PLoS Pathogens·Stanley G SawickiStuart G Siddell
Dec 19, 2013·PLoS Pathogens·Everett Clinton Smith, Mark R Denison
Aug 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano RicagnoBruno Canard
Nov 3, 2014·Annual Review of Virology·Everett Clinton SmithMark R Denison
Jun 12, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kun YuZhiyong Lou
Jul 21, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Youjun Feng, George F Gao
Apr 3, 2007·Virus Research·Rachel L GrahamMark R Denison
Nov 7, 2006·Current Opinion in Structural Biology·Jeroen R MestersRolf Hilgenfeld
Sep 11, 2004·Current Opinion in Microbiology·John Ziebuhr

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.