PMID: 1204611Nov 1, 1975Paper

The sex-factor-dependent exclusion of coli virus T7

European Journal of Biochemistry
H PontaM Schweiger

Abstract

The cause of T7 exclusion by the F episome was investigated. Extracts from neither normal nor infected F+ cells contained an inhibitor of gene expression in vitro. The protein synthesizing systems prepared in vitro from these cells supported T7 early and late protein synthesis with normal efficiency. The content of translational initiation factors in F- and F+ cells, both noninfected and infected, was almost identical. The episome-dependent block of T7 gene expression was observed only in intact cells and detailed kinetics of gene expression in vivo revealed a stop of all transcription and translation at or just before 11 min after T7 infection. The mechanism of F+-dependent T7 exclusion involves both episomal and viral gene products. The data indicate that a T7-induced membrane alteration of the F+ cell membrane leads to cessation of T7 development as well as to the death of the host cell ('suicide').

References

Jun 16, 1975·European Journal of Biochemistry·S H PaiM Schweiger
Apr 1, 1975·European Journal of Biochemistry·M Hirsch-KauffmannH G Wittmann
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Dec 1, 1969·Journal of Virology·D M Molnar, W D Lawton

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Citations

Dec 22, 1976·Molecular & General Genetics : MGG·M Hirsch-KauffmannM Schweiger
Dec 8, 1976·Molecular & General Genetics : MGG·H PontaP Herrlich
Feb 4, 1977·Molecular and Cellular Biochemistry·P HerrlichM Schweiger
Mar 1, 1981·Microbiological Reviews·D H DuckworthD J McCorquodale
Sep 1, 1977·Journal of Virology·W T McAllister, C L Barrett

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