Abstract
Phospholipase C-gamma1 (PLC-gamma1) is a multiple-domain protein and plays an important role in epidermal growth factor (EGF)-induced cell mitogenesis, but the underlying mechanism is unclear. We have previously demonstrated that PLC-gamma1 is required for EGF-induced mitogenesis of squamous cell carcinoma (SCC) cells, but the mitogenic function of PLC-gamma1 is independent of its lipase activity. Earlier studies suggest that the Src homology 3 (SH3) domain of PLC-gamma1 possesses mitogenic activity. In the present study, we sought to determine the role of the SH3 domain of PLC-gamma1 in EGF-induced SCC cell mitogenesis. We examined the effect of overexpression of PLC-gamma1, a catalytically active PLC-gamma1 mutant lacking the SH3 domain or a catalytically inactive PLC-gamma1 mutant lacking the X domain on EGF-induced SCC4 (tongue squamous cell carcinoma) cell mitogenesis. We found that overexpression of PLC-gamma1 enhanced EGF-induced SCC4 cell mitogenesis. This enhancement was abolished by deletion of the SH3 domain but not by deletion of the X catalytic domain. These data suggest that the SH3 domain, but not the catalytic domain, is required for PLC-gamma1 to mediate EGF-induced SCC4 cell mitogenesis.
References
Dec 1, 1991·The Journal of Investigative Dermatology·C K JiangM Blumenberg
May 5, 1989·Science·S G RheeS Y Lee
Dec 19, 1986·Science·P W MajerusD B Wilson
Nov 22, 1984·Nature·M J Berridge, R F Irvine
Jan 30, 1995·FEBS Letters·P S HuangD C Heimbrook
May 6, 1996·Biochemical and Biophysical Research Communications·M R SmithH F Kung
Dec 15, 1996·Structure·R L Williams, M Katan
Jun 13, 1997·The Journal of Biological Chemistry·S G Rhee, Y S Bae
Aug 25, 1997·The Journal of Cell Biology·H q SunH L Yin
Jan 7, 1998·Molecular and Cellular Biology·Z WangM F Moran
Jul 10, 1999·The Journal of Biological Chemistry·Z Xie, D D Bikle
Jul 15, 1999·The International Journal of Biochemistry & Cell Biology·A Wells
Dec 2, 1999·Experimental Cell Research·G Carpenter, Q s Ji
Nov 17, 2001·The Journal of Investigative Dermatology·Z Xie, D D Bikle
Feb 2, 2002·Nature·Keqiang YeSolomon H Snyder
Jan 1, 1993·Trends in Cell Biology·B J Mayer, D Baltimore
Jul 15, 2004·Journal of Cell Science·Jang Hyun ChoiPann-Ghill Suh
May 6, 2005·Molecular Biology of the Cell·Zhongjian XieDaniel D Bikle
Jan 24, 2007·The Journal of Biological Chemistry·Zhongjian Xie, Daniel D Bikle
Jan 23, 2009·Molecular Biology of the Cell·Zhongjian XieDaniel D Bikle
Jun 1, 2010·Biochemical and Biophysical Research Communications·Zhongjian XieSally D Pennypacker
Citations
Aug 9, 2013·Advances in Biological Regulation·Rossano LattanzioMarco Falasca
Jun 19, 2013·Acta Pharmacologica Sinica·Qi Qi, Keqiang Ye
Jul 13, 2017·Journal of the American Heart Association·Dongyang JiangJinjiang Pang
Feb 22, 2012·Oncogene·Z XieS M Chang
May 2, 2020·Journal of Cellular Physiology·Lusha LiZhongjian Xie
Aug 29, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Supratim MandalAdhiraj Roy