The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

The Journal of Cell Biology
Rachel H Roberts-GalbraithKathleen L Gould

Abstract

Schizosaccharomyces pombe cdc15 homology (PCH) family members participate in many cellular processes by bridging the plasma membrane and cytoskeleton. Their F-BAR domains bind and curve membranes, whereas other domains, typically SH3 domains, are expected to provide cytoskeletal links. We tested this prevailing model of functional division in the founding member of the family, Cdc15, which is essential for cytokinesis in S. pombe, and in the related PCH protein, Imp2. We find that the distinct functions of Imp2 and Cdc15 are SH3 domain independent. However, the Cdc15 and Imp2 SH3 domains share an essential role in recruiting proteins to the contractile ring, including Pxl1 and Fic1. Together, Pxl1 and Fic1, a previously uncharacterized C2 domain protein, add structural integrity to the contractile ring and prevent it from fragmenting during division. Our data indicate that the F-BAR proteins Cdc15 and Imp2 contribute to a single biological process with both distinct and overlapping functions.

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Citations

Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Thomas D Pollard, Jian-Qiu Wu
Oct 23, 2012·Molecular Biology of the Cell·Sebastian Mana-CapelliDannel McCollum
Apr 19, 2011·Genes & Development·Franz MeitingerGislene Pereira
Oct 25, 2012·PLoS Genetics·K Adam Bohnert, Kathleen L Gould
Jun 13, 2014·Molecular Biology of the Cell·Nicole RachfallKathleen L Gould
Jul 20, 2011·Cellular and Molecular Life Sciences : CMLS·Yijian Rao, Volker Haucke
Mar 23, 2011·The Journal of Cell Biology·Damien LaporteJian-Qiu Wu
Feb 18, 2015·The Journal of Cell Biology·Alaina H WilletKathleen L Gould
Apr 25, 2012·Seminars in Cell & Developmental Biology·Alexander Hergovich, Brian A Hemmings
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Sep 5, 2015·Current Opinion in Microbiology·Alaina H WilletKathleen L Gould
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Aug 31, 2016·Communicative & Integrative Biology·Juan C G CortésJuan Carlos Ribas
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Jan 9, 2013·Molecular & Cellular Proteomics : MCP·Jun-Song ChenKathleen L Gould
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Jan 1, 2012·Membranes·Fatemeh Safari, Shiro Suetsugu
Aug 4, 2018·Journal of Cell Science·Sumit K Dey, Thomas D Pollard
Sep 16, 2017·ELife·Nathan A McDonaldKathleen L Gould
Jul 6, 2018·Molecular Biology of the Cell·Christine M JonesKathleen L Gould
Jul 29, 2016·Microbiology and Molecular Biology Reviews : MMBR·Juan C García CortésJuan Carlos Ribas

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Methods Mentioned

BETA
two-hybrid
Coimmunoprecipitation
immunoprecipitation
PCR

Software Mentioned

Metamorph
GraphPad
CHIPS
OpenLab
Prism
BLAST

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