PMID: 2488848Jan 1, 1989Paper

The short-term blood biocompatibility of poly(hydroxyethyl methacrylate-co-methyl methacrylate) in an in vitro flow system measured by digital videomicroscopy

Journal of Biomaterials Science. Polymer Edition
N P Desai, J A Hubbell

Abstract

An in vitro flow system for short-term blood biocompatibility testing of solution-castable polymeric biomaterials was developed. This system was relatively free of artefacts resulting from blood contact with materials other than the test material itself. In conjunction with epifluorescence videomicroscopy and digital image processing, this method provided a high resolution, quantitative, continuous analysis of platelet adhesion, aggregation, thrombus formation, and embolization on the biomaterial surface. This system was well suited for performing biochemical assays on post-contact blood for assessment of platelet activation and release as additional measures of the thrombogenicity of the test material. This method for biomaterials evaluation in vitro was demonstrated by a detailed examination of copolymers of hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA). Videomicroscopic analysis of fluorescently labelled platelets adhering per unit area of the polymer surface after 5 min of flow at a wall shear rate of 500 s-1 showed a dramatic decrease with increasing HEMA fraction in the polymer. The release of serotonin and thromboxane A2 by platelets decreased with increasing HEMA fraction. Reflection interference contra...Continue Reading

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Citations

Jun 27, 2007·Journal of Pharmaceutical Sciences·Biancamaria Baroli
Aug 10, 2010·Journal of Biomedical Materials Research. Part a·Daniel E Heath, Stuart L Cooper
Jun 5, 1999·Journal of Biomedical Materials Research·T G van KootenC J Kirkpatrick
Dec 8, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·K S FurukawaT Tateishi
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·Jian YangShenguo Wang
Jun 5, 1999·Journal of Biomedical Materials Research·K FurukawaT Tateishi
Jun 1, 1992·Journal of Biomedical Materials Research·T G van KootenH J Busscher
Nov 1, 1992·Journal of Biomedical Materials Research·S F HossianyJ A Hubbell
Jul 1, 1991·Journal of Biomedical Materials Research·N P Desai, J A Hubbell

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