DOI: 10.1101/453365Nov 5, 2018Paper

The Sicilian wolf: genetic identity of a recently extinct insular population

BioRxiv : the Preprint Server for Biology
Francesco Maria AngeliciE. Cilli

Abstract

During historical times many local grey wolf (Canis lupus) populations underwent a substantial reduction of their sizes or became extinct. Among these, the wolf population once living in Sicily, the biggest island of the Mediterranean Sea, was completely eradicated by human persecution in the early decades of the XX century.\n\nIn order to understand the genetic identity of the Sicilian wolf, we applied ancient DNA techniques to analyse the mitochondrial DNA of six specimens actually stored in Italian museums.\n\nWe successfully amplified a diagnostic mtDNA fragment of the control region (CR) in four of the samples. Results showed that two samples shared the same haplotype, that differed by two substitutions from the currently most diffused Italian wolf haplotype (W14) and one substitution from the only other Italian haplotype (W16). The third sample showed a wolf-like haplotype never described before and the fourth a haplotype commonly found in dogs.\n\nFurthermore, all the wolf haplotypes detected in this study belonged to the mitochondrial haplogroup that includes haplotypes detected in all the known European Pleistocene wolves and in several modern southern European populations.\n\nUnfortunately, this endemic island populat...Continue Reading

Datasets Mentioned

BETA
DQ480509

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Geospiza
PopArt
MEGA7
FIGTREE
DNASP
FinchTV
BLAST
Tracer
MrBayes
Bioedit

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