Pathways of mutagenesis are induced in microbes under adverse conditions controlled by stress responses. Control of mutagenesis by stress responses may accelerate evolution specifically when cells are maladapted to their environments, i.e. are stressed. Stress-induced mutagenesis in the Escherichia coli Lac assay occurs either by 'point' mutation or gene amplification. Point mutagenesis is associated with DNA double-strand-break (DSB) repair and requires DinB error-prone DNA polymerase and the SOS DNA-damage- and RpoS general-stress responses. We report that the RpoE envelope-protein-stress response is also required. In a screen for mutagenesis-defective mutants, we isolated a transposon insertion in the rpoE P2 promoter. The insertion prevents rpoE induction during stress, but leaves constitutive expression intact, and allows cell viability. rpoE insertion and suppressed null mutants display reduced point mutagenesis and maintenance of amplified DNA. Furthermore, sigma(E) acts independently of stress responses previously implicated: SOS/DinB and RpoS, and of sigma(32), which was postulated to affect mutagenesis. I-SceI-induced DSBs alleviated much of the rpoE phenotype, implying that sigma(E) promoted DSB formation. Thus, a th...Continue Reading
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Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance
The rpoE gene of Escherichia coli, which encodes sigma E, is essential for bacterial growth at high temperature
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Adaptive reversion of a frameshift mutation in Escherichia coli by simple base deletions in homopolymeric runs
The activity of sigma E, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins
Modulation of the Escherichia coli sigmaE (RpoE) heat-shock transcription-factor activity by the RseA, RseB and RseC proteins
The sigmaE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of sigmaE
Genome-wide hypermutation in a subpopulation of stationary-phase cells underlies recombination-dependent adaptive mutation
Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA
Mechanisms of mutation in nondividing cells. Insights from the study of adaptive mutation in Escherichia coli
The Escherichia coli sigma(E)-dependent extracytoplasmic stress response is controlled by the regulated proteolysis of an anti-sigma factor
A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors
Different characteristics distinguish early versus late arising adaptive mutations in Escherichia coli FC40
degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide sigma (E) activity
Involvement of sigma(S) in starvation-induced transposition of Pseudomonas putida transposon Tn4652.
DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response
Induction of a DNA nickase in the presence of its target site stimulates adaptive mutation in Escherichia coli.
Adaptive, or stationary-phase, mutagenesis, a component of bacterial differentiation in Bacillus subtilis.
Different spectra of stationary-phase mutations in early-arising versus late-arising mutants of Pseudomonas putida: involvement of the DNA repair enzyme MutY and the stationary-phase sigma factor RpoS.
OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain
Error-prone DNA polymerase IV is controlled by the stress-response sigma factor, RpoS, in Escherichia coli
Impact of a stress-inducible switch to mutagenic repair of DNA breaks on mutation in Escherichia coli
Ecology and evolution as targets: the need for novel eco-evo drugs and strategies to fight antibiotic resistance
Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS
Culture history and population heterogeneity as determinants of bacterial adaptation: the adaptomics of a single environmental transition
What limits the efficiency of double-strand break-dependent stress-induced mutation in Escherichia coli?
High incidence of multiple antibiotic resistant cells in cultures of in enterohemorrhagic Escherichia coli O157:H7
Effect of extremely low frequency magnetic field exposure on DNA transposition in relation to frequency, wave shape and exposure time
Evolution of Escherichia coli for maximum HOCl resistance through constitutive expression of the OxyR regulon
Development of a stress-induced mutagenesis module for autonomous adaptive evolution of Escherichia coli to improve its stress tolerance
Stress-induced mutation via DNA breaks in Escherichia coli: a molecular mechanism with implications for evolution and medicine
Alternative sigma factor σE has an important role in stress tolerance of Yersinia pseudotuberculosis IP32953
Roles of Nucleoid-Associated Proteins in Stress-Induced Mutagenic Break Repair in Starving Escherichia coli
A shifting mutational landscape in 6 nutritional states: Stress-induced mutagenesis as a series of distinct stress input-mutation output relationships
Gross chromosomal rearrangement mediated by DNA replication in stressed cells: evidence from Escherichia coli
Stress-induced mutation rates show a sigmoidal and saturable increase due to the RpoS sigma factor in Escherichia coli
The impact of growth rate and environmental factors on mutation rates and spectra in Escherichia coli
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